pRetroQ-DsRed-Monomer-C1

Self-inactivating retroviral vector for expressing a protein fused to the C-terminus of DsRed-Monomer.

Sequence Author: Clontech (TaKaRa)

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SspI (7228) XmnI (7023) ScaI (6904) AflIII - PciI (5531) BspQI - SapI (5415) AvrII (5284) SfiI (5237) EcoRV (4160) RsrII (3604) CMV enhancer AscI (543) PshAI (677) NheI (2173) BmtI (2177) FspAI (2243) SbfI (2535) NgoMIV (2615) NaeI (2617) PflMI (2776) AleI (2822) PaeR7I - XhoI (2883) BstBI (2897) EcoRI (2899) SalI (2909) AccI (2910) PspOMI (2923) ApaI (2927) BamHI (2930) XbaI * (2942) stop codons BclI * (2952) BlpI (3241) BsmI (3399) BsiWI (3544) pRetroQ-DsRed-Monomer-C1 7573 bp
SspI  (7228)
1 site
A A T A T T T T A T A A
XmnI  (7023)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6904)
1 site
A G T A C T T C A T G A
AflIII  (5531)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5531)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5415)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5415)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (5284)
1 site
C C T A G G G G A T C C
SfiI  (5237)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (4160)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
RsrII  (3604)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AscI  (543)
1 site
G G C G C G C C C C G C G C G G
PshAI  (677)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NheI  (2173)
1 site
G C T A G C C G A T C G
BmtI  (2177)
1 site
G C T A G C C G A T C G
FspAI  (2243)
1 site
R T G C G C A Y Y A C G C G T R
SbfI  (2535)
1 site
C C T G C A G G G G A C G T C C
NgoMIV  (2615)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (2617)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
PflMI  (2776)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (2822)
1 site
C A C N N N N G T G G T G N N N N C A C
PaeR7I  (2883)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2883)
1 site
C T C G A G G A G C T C
BstBI  (2897)
1 site
T T C G A A A A G C T T
EcoRI  (2899)
1 site
G A A T T C C T T A A G
SalI  (2909)
1 site
G T C G A C C A G C T G
AccI  (2910)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PspOMI  (2923)
1 site
G G G C C C C C C G G G
ApaI  (2927)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (2930)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (2942)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (2952)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BlpI  (3241)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsmI  (3399)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsiWI  (3544)
1 site
C G<