pLenti6 BLOCK-iT-DEST
Lentiviral Gateway® destination vector for an shRNA cassette from a U6 entry vector.
Sequence Author: Thermo Fisher (Invitrogen)
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different DraIII sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different CsiI sites may not be compatible. |
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