pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro

Lentiviral expression vector for inducible shRNA expression, with a puromycin resistance marker controlled by the EF-1α promoter.

Sequence Author: Cellecta

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MluI (1) SspI (7503) ScaI (7179) PciI (5806) SfiI (5384) NcoI (5338) AanI (5155) AanI (5099) KpnI (4764) Acc65I (4760) HincII (4165) SalI (4163) DraIII (4128) SexAI * (4124) SphI (96) NruI * (612) BbvCI (1199) AleI (1352) PpuMI - SanDI (1709) BspDI - ClaI (1798) BsaAI (1928) BbsI - BpiI (2065) cloning site BbsI - BpiI (2091) EcoRI (2138) PaeR7I - XhoI (2149) SpeI (2339) 5' LTR (truncated) XbaI (2860) BstXI (2956) BlpI (3414) BclI * (3444) BamHI (3491) BmgBI (3529) PflFI - Tth111I (3599) PasI (3606) BsiWI (3613) BspEI * (3670) RsrII (3673) BstEII (3691) EagI (4005) pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro 8090 bp
MluI  (1)
1 site
A C G C G T T G C G C A
SspI  (7503)
1 site
A A T A T T T T A T A A
ScaI  (7179)
1 site
A G T A C T T C A T G A
PciI  (5806)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SfiI  (5384)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
NcoI  (5338)
1 site
C C