pRetroQ-mCherry-C1

Self-inactivating retroviral vector for expressing a protein fused to the C-terminus of mCherry.

Sequence Author: Clontech (TaKaRa)

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SspI (7260) XmnI (7055) ScaI (6936) FspI (6678) AflIII - PciI (5563) BspQI - SapI (5447) AvrII (5316) SfiI (5269) EcoRV (4192) DraIII (4091) CMV enhancer AscI (542) PshAI (676) NheI (2172) BmtI (2176) SbfI (2549) PflMI (2633) BbvCI (2732) SgrAI (2875) XcmI (2880) BglII (2911) PaeR7I - XhoI (2915) BstBI (2929) EcoRI (2931) SalI (2941) AccI (2942) PspOMI (2955) ApaI (2959) BamHI (2962) XbaI * (2974) stop codons BclI * (2984) BlpI (3273) BsmI (3431) BfuAI - BspMI (3509) BsiWI (3576) RsrII (3636) pRetroQ-mCherry-C1 7605 bp
SspI  (7260)
1 site
A A T A T T T T A T A A
XmnI  (7055)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6936)
1 site
A G T A C T T C A T G A
FspI  (6678)
1 site
T G C G C A A C G C G T
AflIII  (5563)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5563)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5447)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5447)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (5316)
1 site
C C T A G G G G A T C C
SfiI  (5269)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (4192)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
DraIII  (4091)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AscI  (542)
1 site
G G C G C G C C C C G C G C G G
PshAI  (676)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NheI  (2172)
1 site
G C T A G C C G A T C G
BmtI  (2176)
1 site
G C T A G C C G A T C G
SbfI  (2549)
1 site
C C T G C A G G G G A C G T C C
PflMI  (2633)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BbvCI  (2732)
1 site
C C T C A G C G G A G T C G
SgrAI  (2875)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XcmI  (2880)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BglII  (2911)
1 site
A G A T C T T C T A G A
PaeR7I  (2915)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2915)
1 site
C T C G A G G A G C T C
BstBI  (2929)
1 site
T T C G A A A A G C T T
EcoRI  (2931)
1 site
G A A T T C C T T A A G
SalI  (2941)
1 site
G T C G A C C A G C T G
AccI  (2942)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PspOMI  (2955)
1 site
G G G C C C C C C G G G
ApaI  (2959)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BamHI  (2962)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (2974)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (2984)
1 site
T G A T