pLVX-TRE3G-mCherry

Lentiviral vector for doxycycline-inducible expression of a gene together with the mCherry fluorescent protein.

Sequence Author: Clontech (TaKaRa)

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SgrDI (8747) SspI (8630) PvuI (8196) FspI (8048) BmtI (6552) NheI (6548) Bsu36I (5758) CsiI - SexAI * (4987) RsrII (4536) NruI * (833) EcoNI (1170) MfeI (1189) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) SbfI (2919) SgrAI (3245) BsrGI (3264) SfiI (3282) PspOMI (3404) ApaI (3408) AvrII (3442) PaqCI (3630) TspMI - XmaI (3872) SmaI (3874) MluI (3878) NdeI (3885) EcoRI (3890) SphI (3939) AgeI (4010) SpeI (4145) BlpI (4173) PflFI - Tth111I (4462) PasI (4469) BsiWI (4476) pLVX-TRE3G-mCherry 9115 bp
SgrDI  (8747)
1 site
C G T C G A C G G C A G C T G C
SspI  (8630)
1 site
A A T A T T T T A T A A
PvuI  (8196)
1 site
C G A T C G G C T A G C
FspI  (8048)
1 site
T G C G C A A C G C G T
BmtI  (6552)
1 site
G C T A G C C G A T C G
NheI  (6548)
1 site
G C T A G C C G A T C G
Bsu36I  (5758)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
CsiI  (4987)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (4987)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
RsrII  (4536)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
PaeR7I  (2203)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2203)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2203)
1 site
C T C G A G G A G C T C
SbfI  (2919)
1 site
C C T G C A G G G G A C G T C C
SgrAI  (3245)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BsrGI  (3264)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SfiI  (3282)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PspOMI  (3404)
1 site
G G G C C C C C C G G G
ApaI  (3408)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (3442)
1 site
C C T A G G G G A T C C
PaqCI  (3630)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
TspMI  (3872)
1 site
C