pLVX-Tight-Puro
Lentiviral vector for expressing a gene with the Tet-On® Advanced or Tet-Off® Advanced system.
Sequence Author: Clontech (TaKaRa)
Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets
No matches
|
|
|
|
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
PciI is inhibited by nonionic detergents. |
|
|
|
|
| ||
Sticky ends from different Bsu36I sites may not be compatible. |
|
| ||
Sticky ends from different PflMI sites may not be compatible. |
| ||
FseI gradually loses activity when stored at -20°C. |
|
|
|
| ||
Sticky ends from different KflI sites may not be compatible. |
|
|
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|
| ||
* Blocked by Dam methylation. |
|
|
|
|
|
| ||
Sticky ends from different BlpI sites may not be compatible. |
| ||
Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
| ||
Sticky ends from different PasI sites may not be compatible. |
| ||
BsiWI is typically used at 55°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
* Blocked by Dcm methylation. |
| ||
Sticky ends from different CsiI sites may not be compatible. |
| ||
* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
AmpR 6429 .. 7289 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 6429 .. 7220 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 6429 .. 7289 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 7221 .. 7289 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 6429 .. 7289 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
5' LTR 1 .. 634 = 634 bp 5' long terminal repeat (LTR) from HIV-1 |
5' LTR 1 .. 634 = 634 bp 5' long terminal repeat (LTR) from HIV-1 |
3' LTR 4507 .. 5140 = 634 bp 3' long terminal repeat (LTR) from HIV-1 |
3' LTR 4507 .. 5140 = 634 bp 3' long terminal repeat (LTR) from HIV-1 |
PuroR 3096 .. 3695 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 3096 .. 3695 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
WPRE 3712 .. 4300 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
WPRE 3712 .. 4300 = 589 bp woodchuck hepatitis virus posttranscriptional regulatory element |
ori 5670 .. 6258 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 5670 .. 6258 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
PGK promoter 2576 .. 3075 = 500 bp mouse phosphoglycerate kinase 1 promoter |
PGK promoter 2576 .. 3075 = 500 bp mouse phosphoglycerate kinase 1 promoter |
tight TRE promoter 2206 .. 2520 = 315 bp Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter |
tight TRE promoter 2206 .. 2520 = 315 bp Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter |
RRE 1303 .. 1536 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
RRE 1303 .. 1536 = 234 bp The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm. |
SV40 poly(A) signal 7442 .. 7576 = 135 bp SV40 polyadenylation signal |
SV40 poly(A) signal 7442 .. 7576 = 135 bp SV40 polyadenylation signal |
HIV-1 Ψ 681 .. 806 = 126 bp packaging signal of human immunodeficiency virus type 1 |
HIV-1 Ψ 681 .. 806 = 126 bp packaging signal of human immunodeficiency virus type 1 |
cPPT/CTS 2028 .. 2144 = 117 bp central polypurine tract and central termination sequence of HIV-1 (lacking the first T) |
cPPT/CTS 2028 .. 2144 = 117 bp central polypurine tract and central termination sequence of HIV-1 (lacking the first T) |
AmpR promoter 7290 .. 7394 = 105 bp |
AmpR promoter 7290 .. 7394 = 105 bp |
MCS 2528 .. 2571 = 44 bp multiple cloning site |
MCS 2528 .. 2571 = 44 bp multiple cloning site |
tet operator 2214 .. 2232 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2214 .. 2232 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2250 .. 2268 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2250 .. 2268 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2285 .. 2303 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2285 .. 2303 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2321 .. 2339 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 2321 .. 2339 = 1 |