pLVX-Tight-Puro
Lentiviral vector for expressing a gene with the Tet-On® Advanced or Tet-Off® Advanced system.
Sequence Author: Clontech (TaKaRa)
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Sticky ends from different PflMI sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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Sticky ends from different KflI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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| * Blocked by Dam methylation. |
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Sticky ends from different BlpI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different PasI sites may not be compatible. |
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BsiWI is typically used |