pLVX-TRE3G

Lentiviral vector for doxycycline-inducible expression of a gene in cells containing the Tet-On 3G transactivator protein.

Sequence Author: Clontech (TaKaRa)

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XcmI (7676) SgrDI (7451) SspI (7334) PvuI (6900) FspI (6752) AhdI (6530) DrdI (5748) PciI (5640) BmtI (5256) NheI (5252) KpnI (4470) Acc65I (4466) Bsu36I (4462) NcoI (4149) PflMI (3982) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) BamHI (2556) PspOMI (2562) ApaI (2566) NotI (2569) TspMI - XmaI (2576) SmaI (2578) MluI (2582) NdeI (2589) EcoRI (2594) SphI (2643) AgeI (2714) SpeI (2849) BlpI (2877) BfuAI - BspMI (3113) PflFI - Tth111I (3166) PasI (3173) BsiWI (3180) RsrII (3240) BstEII (3258) MscI * (3499) CsiI - SexAI * (3691) pLVX-TRE3G 7819 bp
XcmI  (7676)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (7451)
1 site
C G T C G A C G G C A G C T G C
SspI  (7334)
1 site
A A T A T T T T A T A A
PvuI  (6900)
1 site
C G A T C G G C T A G C
FspI  (6752)
1 site
T G C G C A A C G C G T
AhdI  (6530)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (5748)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PciI  (5640)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BmtI  (5256)
1 site
G C T A G C C G A T C G
NheI  (5252)
1 site
G C T A G C C G A T C G
KpnI  (4470)
1 site
G G T A C C C C A T G G
Acc65I  (4466)
1 site
G G T A C C C C A T G G
Bsu36I  (4462)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4149)
1 site
C C A T G G G G T A C C
PflMI  (3982)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site
A T