pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro

Lentiviral vector for inducible barcoded shRNA libraries, with an HTS4 cassette for high-throughput sequencing plus TagRFP and puromycin resistance markers.

Sequence Author: Cellecta

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HomePlasmidsViral Expression & Packaging VectorspRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
MluI (1) SspI (8119) ScaI (7795) PciI (6422) SfiI (6000) AanI (5771) AanI (5715) KpnI (5380) Acc65I (5376) NaeI (5299) NgoMIV (5297) HincII (4781) SalI (4779) SexAI * (4740) EagI (4621) BstEII (4307) SphI (96) NruI * (612) MfeI (964) BbvCI - Bpu10I (1199) AleI (1352) PpuMI - SanDI (1709) BspDI - ClaI (1798) BbsI - BpiI (2065) cloning site BbsI - BpiI (2091) EcoRI (2118) PaeR7I - PspXI - XhoI (2127) SnaBI (2395) XbaI * (2687) BstXI (2783) BlpI (3241) BclI * (3271) BsrGI (3433) PshAI (4023) NheI (4095) BmtI (4099) PflFI - Tth111I (4215) BsiWI (4229) RsrII (4289) pRSIT12-U6Tet-sh-HTS4-CMV- TetRep-2A-TagRFP-2A-Puro 8706 bp