pRetroX-TRE3G

Retroviral vector for doxycycline-inducible expression of a gene in cells containing the Tet-On® 3G transactivator protein.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspRetroX-TRE3G
CMV enhancer SspI (6255) XmnI (6050) ScaI (5931) PvuI (5821) FspI (5673) PciI (4558) BspQI - SapI (4442) AvrII (4311) SfiI (4264) AccI (3430) SalI (3429) BmtI (3340) NheI (3336) BtgZI (279) SnaBI (285) AscI (543) PshAI (677) AfeI (1089) BglII (1577) PspXI (1605) BamHI (1958) BspDI - ClaI (1965) MluI (1970) NotI (1977) MreI - NgoMIV - SgrAI (1984) NaeI (1986) PspOMI (1990) ApaI (1994) EcoRI (1996) AgeI (2116) BlpI (2279) BsmI (2437) BfuAI - BspMI (2515) BsiWI (2582) RsrII (2642) SacII (2740) DraIII (3097) EcoRV (3194) PvuII (3237) pRetroX-TRE3G 6600 bp
SspI  (6255)
1 site		 A A T A T T T T A T A A
XmnI  (6050)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (5931)
1 site		 A G T A C T T C A T G A
PvuI  (5821)
1 site		 C G A T C G G C T A G C
FspI  (5673)
1 site		 T G C G C A A C G C G T
PciI  (4558)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (4442)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (4442)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (4311)
1 site		 C C T A G G G G A T C C
SfiI  (4264)
1 site		 G G C C N N N N