pCI-neo
Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker.
Sequence Author: Promega
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Sticky ends from different AlwNI sites may not be compatible. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BstXI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different AflIII sites may not be compatible. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
AmpR 3780 .. 4640 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 3780 .. 3848 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3780 .. 4640 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 3849 .. 4640 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3780 .. 4640 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
NeoR/KanR 2463 .. 3257 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
NeoR/KanR 2463 .. 3257 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
ori 4811 .. 5399 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4811 .. 5399 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1483 .. 1938 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1483 .. 1938 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
SV40 promoter 2055 .. 2412 = 358 bp SV40 enhancer and early promoter |
SV40 promoter 2055 .. 2412 = 358 bp SV40 enhancer and early promoter |
CMV enhancer 213 .. 517 = 305 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 213 .. 517 = 305 bp human cytomegalovirus immediate early enhancer |
chimeric intron 890 .. 1022 = 133 bp chimera between introns from human β-globin and immunoglobulin heavy chain genes |
chimeric intron 890 .. 1022 = 133 bp chimera between introns from human β-globin and immunoglobulin heavy chain genes |
SV40 poly(A) signal 1176 .. 1297 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 1176 .. 1297 = 122 bp SV40 polyadenylation signal |
AmpR promoter 3675 .. 3779 = 105 bp |
AmpR promoter 3675 .. 3779 = 105 bp |
MCS 1085 .. 1137 = 53 bp multiple cloning site |
MCS 1085 .. 1137 = 53 bp multiple cloning site |
poly(A) signal 3321 .. 3369 = 49 bp synthetic polyadenylation signal |
poly(A) signal 3321 .. 3369 = 49 bp synthetic polyadenylation signal |
minimal CMV promoter 691 .. 729 = 39 bp human cytomegalovirus (CMV) immediate early promoter |
minimal CMV promoter 691 .. 729 = 39 bp human cytomegalovirus (CMV) immediate early promoter |
T3 promoter 1141 .. 1158 = 18 bp promoter for bacteriophage T3 RNA polymerase (shorter by one base than the standard T3 promoter) |
T3 promoter 1141 .. 1158 = 18 bp promoter for bacteriophage T3 RNA polymerase (shorter by one base than the standard T3 promoter) |
SV40 ori 2263 .. 2398 = 136 bp SV40 origin of replication |
SV40 ori 2263 .. 2398 = 136 bp SV40 origin of replication |
T7 promoter 1067 .. 1085 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 1067 .. 1085 = 19 bp promoter for bacteriophage T7 RNA polymerase |
ORF: 2635 .. 3021 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 2463 .. 3257 = 795 bp ORF: 264 amino acids = 29.0 kDa |
ORF: 3780 .. 4640 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 2772 .. 3308 = 537 bp ORF: 178 amino acids = 19.8 kDa |
ORF: 4244 .. 4510 = 267 bp ORF: 88 amino acids = 9.2 kDa |
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