pSingle-tTs-shRNA

Vector for doxycycline-inducible expression of an shRNA.

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
EcoNI (4) XmnI (6531) PvuI (6302) BsaI (5993) AhdI (5932) Bpu10I (4736) PI-SceI (4661) I-CeuI (4210) PflMI (3912) ApaI (3535) PspOMI (3531) NsiI (61) SfiI (192) StuI (238) BsaBI * (276) EagI (323) KasI (416) NarI (417) SfoI (418) PluTI (420) PflFI - Tth111I (535) RsrII (933) BstBI (1099) BsrGI (1407) SpeI (1463) SnaBI (1804) Eco53kI (2030) SacI (2032) BsmBI - Esp3I (2053) SacII (2119) XbaI (2138) PmlI (2266) NheI (2785) BmtI (2789) BamHI (2971) KflI (3014) MfeI (3356) PsiI (3364) pSingle-tTS-shRNA 6919 bp
EcoNI  (4)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XmnI  (6531)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (6302)
1 site
C G A T C G G C T A G C
BsaI  (5993)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5932)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Bpu10I  (4736)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PI-SceI  (4661)
1 site
A T C T A T G T C G G G T G C G G A G