pSingle-tTs-shRNA

Vector for doxycycline-inducible expression of an shRNA.

Sequence Author: Clontech (TaKaRa)

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EcoNI (4) XmnI (6531) PvuI (6302) BsaI (5993) AhdI (5932) Bpu10I (4736) PI-SceI (4661) I-CeuI (4210) PflMI (3912) ApaI (3535) PspOMI (3531) NsiI (61) SfiI (192) StuI (238) BsaBI * (276) EagI (323) KasI (416) NarI (417) SfoI (418) PluTI (420) PflFI - Tth111I (535) RsrII (933) BstBI (1099) BsrGI (1407) SpeI (1463) SnaBI (1804) Eco53kI (2030) SacI (2032) BsmBI - Esp3I (2053) SacII (2119) XbaI (2138) PmlI (2266) NheI (2785) BmtI (2789) BamHI (2971) KflI (3014) MfeI (3356) PsiI (3364) pSingle-tTS-shRNA 6919 bp
EcoNI  (4)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XmnI  (6531)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (6302)
1 site
C G A T C G G C T A G C
BsaI  (5993)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5932)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Bpu10I  (4736)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PI-SceI  (4661)
1 site
A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C

PI-SceI  is a homing endonuclease that can recognize a variety of similar recognition sequences.
I-CeuI  (4210)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
PflMI  (3912)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
ApaI  (3535)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3531)
1 site
G</