pcDNA6 TR

Mammalian cell vector that yields high-level expression of the tetracycline repressor, for use with the T-REx™ system.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsMammalian Expression VectorspcDNA6 TR
SgrDI (6660) FspI (5961) AhdI (5739) PciI (4849) BspQI - SapI (4733) BstZ17I (4470) PfoI (4273) BlpI (4177) BsaBI * (4080) MscI (3785) CsiI - SexAI * (3420) SpeI (249) CMV enhancer Eco53kI (816) SacI (818) NheI (895) BmtI (899) AflII (908) HindIII (911) Acc65I (917) KpnI (921) BspEI * (1002) BspDI * - ClaI * (1008) KflI (1038) EcoNI (1892) AfeI (2088) AleI (2222) EcoRI (2323) BstXI (2491) NotI (2496) PaeR7I - PspXI - XhoI (2502) SacII (2521) BstBI (2525) AgeI (2575) pcDNA™6/TR 6662 bp
SgrDI  (6660)
1 site		 C G T C G A C G G C A G C T G C
FspI  (5961)
1 site		 T G C G C A A C G C G T
AhdI  (5739)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4849)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (4733)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (4733)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (4470)
1 site		 G T A T A C C A T A T G
PfoI  (4273)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BlpI  (4177)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BsaBI  (4080)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MscI  (3785)
1 site		 T G G C C A A C C G G T
CsiI  (3420)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (3420)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SpeI  (249)
1 site		 A C T A G T T G A T C A
Eco53kI  (816)
1 site		 G A G C T C C T C G A G
SacI  (818)
1 site		 G A G C T C C T C G A G
NheI  (895)
1 site		 G C T A G C C G A T C G
BmtI  (899)
1 site		 G C T A G C C G A T C G
AflII  (908)
1 site		 C T T A A G G A A T T C
HindIII  (911)
1 site		 A A G C T T T T C G A A
Acc65I  (917)
1 site		 G G T A C C C C A T G G
KpnI  (921)
1 site		 G G T A C C C C A T G G
BspEI  (1002)
1 site		 T C C G G A A G G C C T
* Blocked by Dam methylation.
BspDI  (1008)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1008)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
KflI  (1038)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
EcoNI  (1892)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AfeI  (2088)
1 site		 A G C G C T T C G C G A
AleI  (2222)
1 site		 C A C N N N N G T G G T G N N N N C A C
EcoRI  (2323)
1 site		 G A A T T C C T T A A G
BstXI  (2491)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
NotI  (2496)
1 site		 G C G G C C G C C G C C G G C G
PaeR7I  (2502)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2502)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (2502)
1 site		 C T C G A G G A G C T C
SacII  (2521)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstBI  (2525)
1 site		 T T C G A A A A G C T T
AgeI  (2575)
1 site		 A C C G G T T G G C C A
AmpR
5666 .. 6526  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5666 .. 6457  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5666 .. 6526  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6458 .. 6526  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5666 .. 6526  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TetR
1684 .. 2301  =  618 bp
206 amino acids  =  23.3 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
TetR
1684 .. 2301  =  618 bp
206 amino acids  =  23.3 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
ori
4910 .. 5495  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4910 .. 5495  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
1028 .. 1600  =  573 bp
intron from rabbit β-globin gene
β-globin intron
1028 .. 1600  =  573 bp
intron from rabbit β-globin gene
f1 ori
2897 .. 3325  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2897 .. 3325  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BSD
3782 .. 4180  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
3782 .. 4180  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
3339 .. 3668  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
3339 .. 3668  =  330 bp
SV40 enhancer and early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
2346 .. 2467  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2346 .. 2467  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4338 .. 4459  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4338 .. 4459  =  122 bp
SV40 polyadenylation signal
AmpR promoter
6527 .. 6631  =  105 bp
AmpR promoter
6527 .. 6631  =  105 bp
EM7 promoter
3716 .. 3763  =  48 bp
synthetic bacterial promoter
EM7 promoter
3716 .. 3763  =  48 bp
synthetic bacterial promoter
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1655 .. 1673  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1655 .. 1673  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
3519 .. 3654  =  136 bp
SV40 origin of replication
SV40 ori
3519 .. 3654  =  136 bp
SV40 origin of replication
ORF:  1684 .. 2340  =  657 bp
ORF:  218 amino acids  =  24.7 kDa
ORF:  2368 .. 2601  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  2848 .. 3114  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  3782 .. 4180  =  399 bp
ORF:  132 amino acids  =  13.7 kDa
ORF:  5796 .. 6062  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1620 .. 1853  =  234 bp
ORF:  77 amino acids  =  8.4 kDa
ORF:  3728 .. 4084  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  5666 .. 6526  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

p3xFLAG-CMV-10
pcDNA6 myc-His A
pCMV6-AC-YFP
pHet-Act1-2
p3xFLAG-CMV-13
pcDNA6 myc-His B
pCMV6-AN-3DDK
pHet-Mem1
p3xFLAG-CMV-14
pcDNA6 myc-His C
pCMV6-AN-3HA
pHet-Nuc1
p3xFLAG-CMV-7
pcDNA6 TR
pCMV6-AN-DDK
pHom-1
p3xFLAG-CMV-7.1
pcDNA6 V5-His A
pCMV6-AN-FC
pHom-Mem1
p3xFLAG-CMV-8
pcDNA6 V5-His B
pCMV6-AN-FC-S
pHTC HaloTag CMV-neo
p3xFLAG-CMV-9
pcDNA6 V5-His C
pCMV6-AN-FP602
pHTN HaloTag CMV-neo
p3xFLAG-Myc-CMV-23
pCEP4
pCMV6-AN-FP635
pIRES
p3xFLAG-Myc-CMV-24
pCI
pCMV6-AN-GFP
pIRES2 DsRed-Express2
p3xFLAG-Myc-CMV-25
pCI-neo
pCMV6-AN-GFP-AC-His
pIRES2-AcGFP1
p3xFLAG-Myc-CMV-26
pCMV SPORT6
pCMV6-AN-HA
pIRES2-EGFP
pALTER-MAX
pCMV SPORT6.1
pCMV6-AN-His
pIRES2-ZsGreen1
pBApo-CMV
pCMV-(DYKDDDDK)-C
pCMV6-AN-His-DDK
pIRESbleo3
pBApo-CMV Neo
pCMV-(DYKDDDDK)-N
pCMV6-AN-His-HA
pIREShyg3
pBApo-CMV Pur
pCMV-3Tag-1A
pCMV6-AN-His-Myc
pIRESneo3
pBApo-EF1(alpha) Neo
pCMV-3Tag-1B
pCMV6-AN-mBFP
pIRESpuro3
pBApo-EF1(alpha) Pur
pCMV-3Tag-1C
pCMV6-AN-mCFP
pM-secSUMOstar
pBI-CMV1
pCMV-3Tag-2A
pCMV6-AN-mGFP
pM-SUMOstar
pBI-CMV2
pCMV-3Tag-2B
pCMV6-AN-mKate
pME-HA (linearized)
pBI-CMV3
pCMV-3Tag-2C
pCMV6-AN-mRFP
pmRi-mCherry
pBI-CMV4
pCMV-3Tag-3A
pCMV6-AN-Myc
pmRi-ZsGreen1
pBK-CMV
pCMV-3Tag-3B
pCMV6-AN-Myc-DDK
pNTAP-A
pBudCE4.1
pCMV-3Tag-3C
pCMV6-AN-mYFP
pNTAP-B
pcDNA3
pCMV-3Tag-4A
pCMV6-AN-RFP
pNTAP-C
pcDNA3.1+ C-DYK
pCMV-3Tag-4B
pCMV6-AN-YFP
pOG44
pcDNA3.1+ C-HA
pCMV-3Tag-4C
pCMV6-Entry
pPTuner
pcDNA3.1+ C-His
pCMV-3Tag-6
pCMV6-Entry-Cre
pPTuner IRES2
pcDNA3.1+ C-Myc
pCMV-3Tag-7
pCMV6-Entry2
pPTunerC
pcDNA3.1+ N-GST(TEV)
pCMV-3Tag-8
pCMV6-XL5-DDK-His
pREP4
pcDNA3.1+ N-GST(Thrombin)
pCMV-3Tag-9
pCS6-Empty
prHom-1
pcDNA3.1+ N-HA
pCMV-DDK-C
pCTAP-A
prHom-Nuc1
pcDNA3.1+ N-His
pCMV-DDK-N
pCTAP-B
prHom-Sec1
pcDNA3.1+ N-Myc
pCMV-HA
pCTAP-C
pSecTag A
pcDNA-DEST40
pCMV-HA-C
pDEST26
pSecTag B
pcDNA-DEST47
pCMV-HA-N
pDEST27
pSecTag C
pcDNA-DEST53
pCMV-LacZ
pDisplay
pSecTag2 A
pcDNA3.1(+)
pCMV-MIR
pEF-DEST51
pSecTag2 B
pcDNA3.1(-)
pCMV-Myc
pEF1alpha-IRES
pSecTag2 C
pcDNA3.1 CT-GFP
pCMV-Myc-C
pEF1alpha-IRES-AcGFP1
pSI
pcDNA3.1 His A
pCMV-Myc-N
pEF1alpha-IRES-DsRed-Express2
pSingle-tTs-shRNA
pcDNA3.1 His B
pCMV-Script
pEF1alpha-IRES-ZsGreen1
pT-REx-DEST30
pcDNA3.1 His C
pCMV-Script EX
pEF1alpha-Tet3G
pT-REx-DEST31
pcDNA3.1 Hygro(+)
pCMV-Tag 1
pEF5 FRT V5-DEST
pTandem-1
pcDNA3.1 Hygro(-)
pCMV-Tag 2A
pEF6 V5-His A
pTCN
pcDNA3.1 myc-His A
pCMV-Tag 2B
pEF6 V5-His B
pTCP
pcDNA3.1 myc-His B
pCMV-Tag 2C
pEF6 V5-His C
pTet-DualOFF
pcDNA3.1 myc-His C
pCMV-Tag 3A
pEntry-rtTA
pTet-DualON
pcDNA3.1 myc-His(-) A
pCMV-Tag 3B
pF4A CMV
pTet-Off
pcDNA3.1 myc-His(-) B
pCMV-Tag 3C
pF4K CMV
pTet-Off-Advanced
pcDNA3.1 myc-His(-) C
pCMV-Tag 4A
pF5A CMV-neo
pTet-On
pcDNA3.1 NT-GFP
pCMV-Tag 4B
pF5K CMV-neo
pTet-On-Advanced
pcDNA3.1 nV5-DEST
pCMV-Tag 4C
pFC14A HaloTag CMV
pTet-tTS
pcDNA3.1 V5-His A
pCMV-Tag 5A
pFC14K HaloTag CMV
pTetOne
pcDNA3.1 V5-His B
pCMV-Tag 5B
pFC15A HaloTag CMVd1
pTK-neo
pcDNA3.1 V5-His C
pCMV-Tag 5C
pFC15K HaloTag CMVd1
pTracer-CMV
pcDNA3.1 Zeo(+)
pCMV-Tet3G
pFC16A HaloTag CMVd2
pTracer-CMV Bsd
pcDNA3.1 Zeo(-)
pCMV6-A-BSD
pFC16K HaloTag CMVd2
pTracer-CMV2
pcDNA3.2 V5-DEST
pCMV6-A-EM7-BSD
pFC17A HaloTag CMVd3
pTracer-EF V5-His A
pcDNA4 His A
pCMV6-A-GFP
pFC17K HaloTag CMVd3
pTracer-EF V5-His B
pcDNA4 His B
pCMV6-A-Hygro
pFC27A HaloTag CMV-neo
pTracer-EF V5-His C
pcDNA4 His C
pCMV6-A-Puro
pFC27K HaloTag CMV-neo
pTracer-SV40
pcDNA4 HisMax A
pCMV6-AC
pFLAG-CMV-1
pTRE-Cycle1
pcDNA4 HisMax B
pCMV6-AC-3DDK
pFLAG-CMV-2
pTRE-Cycle2
pcDNA4 HisMax C
pCMV6-AC-3HA
pFLAG-CMV-3
pTRE-Cycle3
pcDNA4 myc-His A
pCMV6-AC-DDK
pFLAG-CMV-4
pTRE-Dual1
pcDNA4 myc-His B
pCMV6-AC-DDK-His
pFLAG-CMV-5.1
pTRE-Dual2
pcDNA4 myc-His C
pCMV6-AC-FC
pFLAG-Myc-CMV-19
pTRE-Tight
pcDNA4 TO
pCMV6-AC-FC-S
pFLAG-Myc-CMV-20
pTRE-Tight-BI
pcDNA4 TO myc-His A
pCMV6-AC-FP602
pFLAG-Myc-CMV-21
pTRE-Tight-BI-AcGFP1
pcDNA4 TO myc-His B
pCMV6-AC-FP635
pFLAG-Myc-CMV-22
pTRE-Tight-BI-DsRed-Express
pcDNA4 TO myc-His C
pCMV6-AC-GFP
pFN21A HaloTag CMV
pTRE-Tight-BI-ZsGreen1
pcDNA4 V5-His A
pCMV6-AC-GFP-rtTA
pFN21K HaloTag CMV
pTRE2
pcDNA4 V5-His B
pCMV6-AC-HA
pFN22A HaloTag CMVd1
pTRE2hyg
pcDNA4 V5-His C
pCMV6-AC-HA-His
pFN22K HaloTag CMVd1
pTRE2pur
pcDNA5 FRT
pCMV6-AC-His
pFN23A HaloTag CMVd2
pTRE3G
pcDNA5 FRT TO
pCMV6-AC-IRES-GFP
pFN23K HaloTag CMVd2
pTRE3G-BI
pcDNA5 TO
pCMV6-AC-IRES-GFP-Puro
pFN24A HaloTag CMVd3
pTRE3G-BI-Het1
pcDNA6.2 C-EmGFP-DEST
pCMV6-AC-mBFP
pFN24K HaloTag CMVd3
pTRE3G-BI-mCherry
pcDNA6.2 C-YFP-DEST
pCMV6-AC-mCFP
pFN28A HaloTag CMV-neo
pTRE3G-BI-ZsGreen1
pcDNA6.2 cGeneBLAzer-DEST
pCMV6-AC-mGFP
pFN28K HaloTag CMV-neo
pTRE3G-IRES
pcDNA6.2 cLumio-DEST
pCMV6-AC-mKate
pFRT lacZeo
pTRE3G-mCherry
pcDNA6.2 EmGFP-Bsd V5-DEST
pCMV6-AC-mRFP
pFRT lacZeo2
pTRE3G-ZsGreen1