pcDNA6 TR

Mammalian cell vector that yields high-level expression of the tetracycline repressor, for use with the T-REx™ system.

Sequence Author: Thermo Fisher (Invitrogen)

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SgrDI (6660) FspI (5961) AhdI (5739) PciI (4849) BspQI - SapI (4733) BstZ17I (4470) PfoI (4273) BlpI (4177) BsaBI * (4080) MscI (3785) CsiI - SexAI * (3420) SpeI (249) CMV enhancer Eco53kI (816) SacI (818) NheI (895) BmtI (899) AflII (908) HindIII (911) Acc65I (917) KpnI (921) BspEI * (1002) BspDI * - ClaI * (1008) KflI (1038) EcoNI (1892) AfeI (2088) AleI (2222) EcoRI (2323) BstXI (2491) NotI (2496) PaeR7I - PspXI - XhoI (2502) SacII (2521) BstBI (2525) AgeI (2575) pcDNA™6/TR 6662 bp
SgrDI  (6660)
1 site
C G T C G A C G G C A G C T G C
FspI  (5961)
1 site
T G C G C A A C G C G T
AhdI  (5739)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4849)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (4733)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4733)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (4470)
1 site
G T A T A C C A T A T G
PfoI  (4273)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BlpI  (4177)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsaBI  (4080)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MscI  (3785)
1 site
T G G C C A A C C G G T
CsiI  (3420)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (3420)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SpeI  (249)
1 site
A C T A G T T G A T C A
Eco53kI  (816)
1 site
G A G C T C C T C G A G
SacI  (818)
1 site
G A G C T C C T C G A G
NheI  (895)
1 site
G C T A G C C G A T C G
BmtI  (899)
1 site
G C T A G C C G A T C G
AflII  (908)
1 site
C T T A A G G A A T T C
HindIII  (911)
1 site
A A G C T T T T C G A A
Acc65I  (917)
1 site
G G T A C C C C A T G G
KpnI  (921)
1 site
G G T A C C C C A T G G
BspEI  (1002)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
BspDI  (1008)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1008)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
KflI  (1038)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
EcoNI  (1892)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AfeI  (2088)
1 site
A G C G C T T C G C G A
AleI  (2222)
1 site
C A C N N N N G T G G T G N N N N C A C
EcoRI  (2323)
1 site
G A A T T C C T T A A G
BstXI  (2491)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (2496)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (2502)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2502)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2502)
1 site
C T C G A G G A G C T C
SacII  (2521)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstBI  (2525)
1 site
T T C G A A A A G C T T
AgeI  (2575)
1 site
A C C G G T T G G C C A
AmpR
5666 .. 6526  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5666 .. 6457  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5666 .. 6526  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6458 .. 6526  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5666 .. 6526  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TetR
1684 .. 2301  =  618 bp
206 amino acids  =  23.3 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
TetR
1684 .. 2301  =  618 bp
206 amino acids  =  23.3 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
ori
4910 .. 5495  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4910 .. 5495  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
1028 .. 1600  =  573 bp
intron from rabbit β-globin gene
β-globin intron
1028 .. 1600  =  573 bp
intron from rabbit β-globin gene
f1 ori
2897 .. 3325  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2897 .. 3325  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BSD
3782 .. 4180  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
3782 .. 4180  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
3339 .. 3668  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
3339 .. 3668  =  330 bp
SV40 enhancer and early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
2346 .. 2467  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2346 .. 2467  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4338 .. 4459  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4338 .. 4459  =  122 bp
SV40 polyadenylation signal
AmpR promoter
6527 .. 6631  =  105 bp
AmpR promoter
6527 .. 6631  =  105 bp
EM7 promoter
3716 .. 3763  =  48 bp
synthetic bacterial promoter
EM7 promoter
3716 .. 3763  =  48 bp
synthetic bacterial promoter
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1655 .. 1673  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1655 .. 1673  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
3519 .. 3654  =  136 bp
SV40 origin of replication
SV40 ori
3519 .. 3654  =  136 bp
SV40 origin of replication
ORF:  1684 .. 2340  =  657 bp
ORF:  218 amino acids  =  24.7 kDa
ORF:  2368 .. 2601  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  2848 .. 3114  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  3782 .. 4180  =  399 bp
ORF:  132 amino acids  =  13.7 kDa
ORF:  5796 .. 6062  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1620 .. 1853  =  234 bp
ORF:  77 amino acids  =  8.4 kDa
ORF:  3728 .. 4084  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  5666 .. 6526  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download pcDNA6 TR.dna file

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