pCMV6-Entry-Cre

Mammalian vector for expressing myc- and FLAG®-tagged Cre to enable Cre-Lox recombination.

Sequence Author: OriGene

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DraIII (5713) SfiI (5054) StuI (5005) PluTI (4827) SfoI (4825) NarI (4824) KasI (4823) PflFI - Tth111I (4707) ApaLI (3218) BsrGI (301) SpeI (357) AseI (365) CMV enhancer NdeI (592) Eco53kI (924) SacI (926) EcoRI (979) SalI (985) AccI (986) Acc65I (998) KpnI (1002) BglII (1008) XmnI (1237) NruI (1243) BssHII (1266) RsrII (1330) AclI (1399) PaqCI (1638) PshAI (1864) MluI (2058) BsiWI (2061) NotI (2067) PaeR7I - PspXI - XhoI (2073) PmeI (2151) FseI (2161) SacII (2165) TspMI - XmaI (2191) SmaI (2193) AleI (2251) AhdI (2307) BbsI (2374) BsgI (2435) BbvCI (2477) BlpI (2521) BstXI (2628) PflMI (2742) pCMV6-Entry-Cre 5910 bp
DraIII  (5713)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (5054)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (5005)
1 site
A G G C C T T C C G G A
PluTI  (4827)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4825)
1 site
G G C G C C C C G C G G
NarI  (4824)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4823)
1 site
G G C G C C C C G C G G
PflFI  (4707)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4707)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
ApaLI  (3218)
1 site
G T G C A C C A C G T G
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (357)
1 site
A C T A G T T G A T C A
AseI  (365)
1 site
A T T A A T T A A T T A
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
SalI  (985)
1 site
G T C G A C C A G C T G
AccI  (986)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
BglII  (1008)
1 site
A G A T C T T C T A G A
XmnI  (1237)
1 site
G A A N N N N T T C C T T N N N N A A G
NruI  (1243)
1 site
T C G C G A A G C G C T
BssHII  (1266)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (1330)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AclI  (1399)
1 site
A A C G T T T T G C A A
PaqCI  (1638)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
PshAI  (1864)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MluI  (2058)
1 site
A C G C G T T G C G C A
BsiWI  (2061)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NotI  (2067)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (2073)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2073)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2073)
1 site
C T C G A G G A G C T C
PmeI  (2151)
1 site
G T T T A A A C C A A A T T T G
FseI  (2161)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SacII  (2165)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
TspMI  (2191)
1 site
C C C G G G G G G C C C
XmaI  (2191)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2193)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
AleI  (2251)
1 site
C A C N N N N G T G G T G N N N N C A C
AhdI  (2307)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BbsI  (2374)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsgI  (2435)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbvCI  (2477)
1 site
C C T C A G C G G A G T C G
BlpI  (2521)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BstXI  (2628)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PflMI  (2742)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Cre
1029 .. 2057  =  1029 bp
343 amino acids  =  38.5 kDa
Product: site-specific recombinase
Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences.
Cre
1029 .. 2057  =  1029 bp
343 amino acids  =  38.5 kDa
Product: site-specific recombinase
Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences.
Myc
2076 .. 2105  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
2076 .. 2105  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
FLAG
2124 .. 2147  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
2124 .. 2147  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
NeoR/KanR
4161 .. 4955  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4161 .. 4955  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
hGH poly(A) signal
2194 .. 2816  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
2194 .. 2816  =  623 bp
human growth hormone polyadenylation signal
ori
2965 .. 3553  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2965 .. 3553  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5480 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5480 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
4990 .. 5347  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
4990 .. 5347  =  358 bp
SV40 enhancer and early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
5349 .. 5453  =  105 bp
AmpR promoter
5349 .. 5453  =  105 bp
HSV TK poly(A) signal
3882 .. 3929  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
3882 .. 3929  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
5004 .. 5139  =  136 bp
SV40 origin of replication
SV40 ori
5004 .. 5139  =  136 bp
SV40 origin of replication
ORF:  882 .. 2153  =  1272 bp
ORF:  423 amino acids  =  47.8 kDa
ORF:  3702 .. 3935  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  4110 .. 4646  =  537 bp
ORF:  178 amino acids  =  19.9 kDa
ORF:  3691 .. 4140  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  4161 .. 4955  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2558 .. 2782  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  4397 .. 4783  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
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