pFC17A HaloTag CMVd3

Flexi® vector with an ampicillin resistance marker, for mammalian expression from a strongly compromised CMV promoter of a protein with a cleavable C-terminal HaloTag®.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
BsaBI * (4121) MluI (4003) BspEI (3790) PciI (3026) AgeI (2958) BstBI (2931) AseI (2681) FspI (2633) XmnI (2256) BglII (4122) HindIII (72) AsiSI - PvuI - SgfI (131) BtgI - NcoI (134) Eco53kI - EcoICRI (515) SacI (517) PaeR7I - XhoI (518) PflMI (604) AleI (662) BstEII (680) BseRI (682) PflFI - Tth111I (812) BmgBI (814) PasI (844) StuI * (1013) BclI * (1044) BspDI * - ClaI * (1050) PshAI (1109) SgrAI (1196) BbsI (1395) BssHII (1421) XbaI (1463) SalI (1469) PstI - SbfI (1479) BfuAI - BspMI (1482) BlpI (1544) NotI (1574) AanI - PsiI (1697) HpaI (1717) MfeI (1726) SnaBI (1918) SspI (2051) pFC17A HaloTag® CMVd3 4127 bp
BsaBI  (4121)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MluI  (4003)
1 site
A C G C G T T G C G C A
BspEI  (3790)
1 site
T C C G G A A G G C C T
PciI  (3026)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (2958)
1 site
A C C G G T T G G C C A
BstBI  (2931)
1 site
T T C G A A A A G C T T
AseI  (2681)
1 site
A T T A A T T A A T T A
FspI  (2633)
1 site
T G C G C A A C G C G T
XmnI  (2256)
1 site
G A A N N N N T T C C T T N N N N A A G
BglII  (4122)
1 site
A G A T C T T C T A G A
HindIII  (72)
1 site
A A G C T T T T C G A A
AsiSI  (131)
1 site
G C G A T C G C C G C T A G C G
PvuI  (131)
1 site
C G A T C G G C T A G C
SgfI  (131)
1 site
G C G A T C G C C G C T A G C G
BtgI  (134)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (134)
1 site
C C A T G G G G T A C C
Eco53kI  (515)
1 site
G A G C T C C T C G A G
EcoICRI  (515)
1 site
G A G C T C C T C G A G
SacI  (517)
1 site
G A G C T C C T C G A G
PaeR7I  (518)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (518)
1 site
C T C G A G G A G C T C
PflMI  (604)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (662)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (680)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BseRI  (682)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflFI  (812)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (812)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmgBI  (814)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PasI  (844)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
StuI  (1013)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BclI  (1044)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspDI  (1050)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1050)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PshAI  (1109)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SgrAI  (1196)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BbsI  (1395)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BssHII  (1421)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
XbaI  (1463)
1 site
T C T A G A A G A T C T
SalI  (1469)
1 site
G T C G A C C A G C T G
PstI  (1479)
1 site
C T G C A G G A C G T C
SbfI  (1479)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (1482)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1482)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (1544)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NotI  (1574)
1 site
G C G G C C G C C G C C G G C G
AanI  (1697)
1 site
T T A T A A A A T A T T
PsiI  (1697)
1 site
T T A T A A A A T A T T
HpaI  (1717)
1 site
G T T A A C C A A T T G
MfeI  (1726)
1 site
C A A T T G G T T A A C
SnaBI  (1918)
1 site
T A C G T A A T G C A T
SspI  (2051)
1 site
A A T A T T T T A T A A
TEV site
533 .. 553  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
TEV site
533 .. 553  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
HaloTag®
569 .. 1450  =  882 bp
294 amino acids  =  33.4 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
HaloTag®
569 .. 1450  =  882 bp
294 amino acids  =  33.4 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
AmpR
2069 .. 2929  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2069 .. 2137  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2069 .. 2929  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2138 .. 2929  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2069 .. 2929  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
3087 .. 3675  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3087 .. 3675  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
158 .. 493  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
158 .. 493  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
3791 .. 4074  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
3791 .. 4074  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
SV40 poly(A) signal
1596 .. 1717  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1596 .. 1717  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1964 .. 2068  =  105 bp
AmpR promoter
1964 .. 2068  =  105 bp
CMVd3 promoter
1 .. 45  =  45 bp
truncated human cytomegalovirus (CMV) immediate early promoter that yields strongly reduced expression relative to the full-length promoter
CMVd3 promoter
1 .. 45  =  45 bp
truncated human cytomegalovirus (CMV) immediate early promoter that yields strongly reduced expression relative to the full-length promoter
T7 promoter
77 .. 95  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
77 .. 95  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
101 .. 119  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
101 .. 119  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  1822 .. 2172  =  351 bp
ORF:  116 amino acids  =  13.1 kDa
ORF:  158 .. 493  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  500 .. 1456  =  957 bp
ORF:  318 amino acids  =  36.0 kDa
ORF:  2069 .. 2929  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  497 .. 1126  =  630 bp
ORF:  209 amino acids  =  23.0 kDa
ORF:  1126 .. 1362  =  237 bp
ORF:  78 amino acids  =  8.0 kDa
ORF:  2533 .. 2799  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pFC17A HaloTag CMVd3.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps