prHom-Sec1

Mammalian vector encoding four FKBP homodimerizer domains, for creating ER-localized oligomers that can be dissociated with a drug. Also known as pC4S1-FM4-FCS-hGH.

Sequence Author: Clontech (TaKaRa)

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NaeI (6688) NgoMIV (6686) DraIII (6585) BsaI (5186) AhdI (5125) BspQI - SapI (4116) SfiI (3931) StuI (3882) AvrII (3877) AbsI - PaeR7I - PspXI - XhoI (3854) PflMI (3611) MluI (1) CMV enhancer SnaBI (298) EcoRI (656) XbaI (770) SpeI (2078) AflII (2119) PstI (2241) KasI (2414) NarI (2415) SfoI (2416) PluTI (2418) PflFI - Tth111I (2618) BamHI (2685) KflI (2714) HincII (2866) MfeI (3056) PspOMI (3231) ApaI (3235) MscI (3376) prHom-Sec1 7002 bp
NaeI  (6688)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6686)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (6585)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaI  (5186)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5125)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (4116)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4116)
1 site
G C T C T T C N C G A G A A G N N N N