prHom-Sec1

Mammalian vector encoding four FKBP homodimerizer domains, for creating ER-localized oligomers that can be dissociated with a drug. Also known as pC4S1-FM4-FCS-hGH.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsMammalian Expression VectorsprHom-Sec1
NaeI (6688) NgoMIV (6686) DraIII (6585) BsaI (5186) AhdI (5125) BspQI - SapI (4116) SfiI (3931) StuI (3882) AvrII (3877) AbsI - PaeR7I - PspXI - XhoI (3854) PflMI (3611) MluI (1) CMV enhancer SnaBI (298) EcoRI (656) XbaI (770) SpeI (2078) AflII (2119) PstI (2241) KasI (2414) NarI (2415) SfoI (2416) PluTI (2418) PflFI - Tth111I (2618) BamHI (2685) KflI (2714) HincII (2866) MfeI (3056) PspOMI (3231) ApaI (3235) MscI (3376) prHom-Sec1 7002 bp
NaeI  (6688)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6686)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (6585)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsaI  (5186)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5125)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (4116)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (4116)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SfiI  (3931)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (3882)
1 site		 A G G C C T T C C G G A
AvrII  (3877)
1 site		 C C T A G G G G A T C C
AbsI  (3854)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (3854)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3854)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (3854)
1 site		 C T C G A G G A G C T C
PflMI  (3611)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
MluI  (1)
1 site		 A C G C G T T G C G C A
SnaBI  (298)
1 site		 T A C G T A A T G C A T
EcoRI  (656)
1 site		 G A A T T C C T T A A G
XbaI  (770)
1 site		 T C T A G A A G A T C T
SpeI  (2078)
1 site		 A C T A G T T G A T C A
AflII  (2119)
1 site		 C T T A A G G A A T T C
PstI  (2241)
1 site		 C T G C A G G A C G T C
KasI  (2414)
1 site		 G G C G C C C C G C G G
NarI  (2415)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (2416)
1 site		 G G C G C C C C G C G G
PluTI  (2418)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
PflFI  (2618)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2618)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BamHI  (2685)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KflI  (2714)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
HincII  (2866)
1 site		 G T Y R A C C A R Y T G
MfeI  (3056)
1 site		 C A A T T G G T T A A C
PspOMI  (3231)
1 site		 G G G C C C C C C G G G
ApaI  (3235)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
MscI  (3376)
1 site		 T G G C C A A C C G G T
hGH signal sequence
692 .. 769  =  78 bp
26 amino acids  =  2.7 kDa
Product: human growth hormone signal sequence
hGH signal sequence
692 .. 769  =  78 bp
26 amino acids  =  2.7 kDa
Product: human growth hormone signal sequence
DmrD
776 .. 1096  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
776 .. 1096  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1103 .. 1423  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1103 .. 1423  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1430 .. 1750  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1430 .. 1750  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1757 .. 2077  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1757 .. 2077  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding protein FKBP12
dimerization can be reversed by ligand binding
furin cleavage site
2087 .. 2104  =  18 bp
6 amino acids  =  857.0 Da
Product: cleavage site for the furin protease in the trans-Golgi network
furin cleavage site
2087 .. 2104  =  18 bp
6 amino acids  =  857.0 Da
Product: cleavage site for the furin protease in the trans-Golgi network
stuffer sequence
2105 .. 2677  =  573 bp
191 amino acids  =  22.1 kDa
to be replaced by the coding sequence of interest
stuffer sequence
2105 .. 2677  =  573 bp
191 amino acids  =  22.1 kDa
to be replaced by the coding sequence of interest
AmpR
5052 .. 5924  =  873 bp
290 amino acids  =  32.0 kDa
2 segments
   Segment 2:  
   5052 .. 5855  =  804 bp
   267 amino acids  =  29.4 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5052 .. 5924  =  873 bp
290 amino acids  =  32.0 kDa
2 segments
   Segment 1:  signal sequence  
   5856 .. 5924  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5052 .. 5924  =  873 bp
290 amino acids  =  32.0 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4293 .. 4881  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4293 .. 4881  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
2704 .. 3276  =  573 bp
intron from rabbit β-globin gene
β-globin intron
2704 .. 3276  =  573 bp
intron from rabbit β-globin gene
f1 ori
6361 .. 6816  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6361 .. 6816  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 promoter
3861 .. 4056  =  196 bp
SV40 promoter
3861 .. 4056  =  196 bp
AmpR promoter
5925 .. 6029  =  105 bp
AmpR promoter
5925 .. 6029  =  105 bp
β-globin poly(A) signal
3473 .. 3528  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
3473 .. 3528  =  56 bp
rabbit β-globin polyadenylation signal
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus thymidine kinase gene
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus thymidine kinase gene
SV40 ori
3881 .. 4016  =  136 bp
SV40 origin of replication
SV40 ori
3881 .. 4016  =  136 bp
SV40 origin of replication
ORF:  5182 .. 5448  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  692 .. 2680  =  1989 bp
ORF:  662 amino acids  =  74.1 kDa
ORF:  6832 .. 105  =  276 bp
ORF:  91 amino acids  =  9.5 kDa
ORF:  5052 .. 5924  =  873 bp
ORF:  290 amino acids  =  32.0 kDa
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Individual Sequences & Maps

p3xFLAG-CMV-10
pcDNA6 myc-His A
pCMV6-AC-YFP
pHet-Act1-2
p3xFLAG-CMV-13
pcDNA6 myc-His B
pCMV6-AN-3DDK
pHet-Mem1
p3xFLAG-CMV-14
pcDNA6 myc-His C
pCMV6-AN-3HA
pHet-Nuc1
p3xFLAG-CMV-7
pcDNA6 TR
pCMV6-AN-DDK
pHom-1
p3xFLAG-CMV-7.1
pcDNA6 V5-His A
pCMV6-AN-FC
pHom-Mem1
p3xFLAG-CMV-8
pcDNA6 V5-His B
pCMV6-AN-FC-S
pHTC HaloTag CMV-neo
p3xFLAG-CMV-9
pcDNA6 V5-His C
pCMV6-AN-FP602
pHTN HaloTag CMV-neo
p3xFLAG-Myc-CMV-23
pCEP4
pCMV6-AN-FP635
pIRES
p3xFLAG-Myc-CMV-24
pCI
pCMV6-AN-GFP
pIRES2 DsRed-Express2
p3xFLAG-Myc-CMV-25
pCI-neo
pCMV6-AN-GFP-AC-His
pIRES2-AcGFP1
p3xFLAG-Myc-CMV-26
pCMV SPORT6
pCMV6-AN-HA
pIRES2-EGFP
pALTER-MAX
pCMV SPORT6.1
pCMV6-AN-His
pIRES2-ZsGreen1
pBApo-CMV
pCMV-(DYKDDDDK)-C
pCMV6-AN-His-DDK
pIRESbleo3
pBApo-CMV Neo
pCMV-(DYKDDDDK)-N
pCMV6-AN-His-HA
pIREShyg3
pBApo-CMV Pur
pCMV-3Tag-1A
pCMV6-AN-His-Myc
pIRESneo3
pBApo-EF1(alpha) Neo
pCMV-3Tag-1B
pCMV6-AN-mBFP
pIRESpuro3
pBApo-EF1(alpha) Pur
pCMV-3Tag-1C
pCMV6-AN-mCFP
pM-secSUMOstar
pBI-CMV1
pCMV-3Tag-2A
pCMV6-AN-mGFP
pM-SUMOstar
pBI-CMV2
pCMV-3Tag-2B
pCMV6-AN-mKate
pME-HA (linearized)
pBI-CMV3
pCMV-3Tag-2C
pCMV6-AN-mRFP
pmRi-mCherry
pBI-CMV4
pCMV-3Tag-3A
pCMV6-AN-Myc
pmRi-ZsGreen1
pBK-CMV
pCMV-3Tag-3B
pCMV6-AN-Myc-DDK
pNTAP-A
pBudCE4.1
pCMV-3Tag-3C
pCMV6-AN-mYFP
pNTAP-B
pcDNA3
pCMV-3Tag-4A
pCMV6-AN-RFP
pNTAP-C
pcDNA3.1+ C-DYK
pCMV-3Tag-4B
pCMV6-AN-YFP
pOG44
pcDNA3.1+ C-HA
pCMV-3Tag-4C
pCMV6-Entry
pPTuner
pcDNA3.1+ C-His
pCMV-3Tag-6
pCMV6-Entry-Cre
pPTuner IRES2
pcDNA3.1+ C-Myc
pCMV-3Tag-7
pCMV6-Entry2
pPTunerC
pcDNA3.1+ N-GST(TEV)
pCMV-3Tag-8
pCMV6-XL5-DDK-His
pREP4
pcDNA3.1+ N-GST(Thrombin)
pCMV-3Tag-9
pCS6-Empty
prHom-1
pcDNA3.1+ N-HA
pCMV-DDK-C
pCTAP-A
prHom-Nuc1
pcDNA3.1+ N-His
pCMV-DDK-N
pCTAP-B
prHom-Sec1
pcDNA3.1+ N-Myc
pCMV-HA
pCTAP-C
pSecTag A
pcDNA-DEST40
pCMV-HA-C
pDEST26
pSecTag B
pcDNA-DEST47
pCMV-HA-N
pDEST27
pSecTag C
pcDNA-DEST53
pCMV-LacZ
pDisplay
pSecTag2 A
pcDNA3.1(+)
pCMV-MIR
pEF-DEST51
pSecTag2 B
pcDNA3.1(-)
pCMV-Myc
pEF1alpha-IRES
pSecTag2 C
pcDNA3.1 CT-GFP
pCMV-Myc-C
pEF1alpha-IRES-AcGFP1
pSI
pcDNA3.1 His A
pCMV-Myc-N
pEF1alpha-IRES-DsRed-Express2
pSingle-tTs-shRNA
pcDNA3.1 His B
pCMV-Script
pEF1alpha-IRES-ZsGreen1
pT-REx-DEST30
pcDNA3.1 His C
pCMV-Script EX
pEF1alpha-Tet3G
pT-REx-DEST31
pcDNA3.1 Hygro(+)
pCMV-Tag 1
pEF5 FRT V5-DEST
pTandem-1
pcDNA3.1 Hygro(-)
pCMV-Tag 2A
pEF6 V5-His A
pTCN
pcDNA3.1 myc-His A
pCMV-Tag 2B
pEF6 V5-His B
pTCP
pcDNA3.1 myc-His B
pCMV-Tag 2C
pEF6 V5-His C
pTet-DualOFF
pcDNA3.1 myc-His C
pCMV-Tag 3A
pEntry-rtTA
pTet-DualON
pcDNA3.1 myc-His(-) A
pCMV-Tag 3B
pF4A CMV
pTet-Off
pcDNA3.1 myc-His(-) B
pCMV-Tag 3C
pF4K CMV
pTet-Off-Advanced
pcDNA3.1 myc-His(-) C
pCMV-Tag 4A
pF5A CMV-neo
pTet-On
pcDNA3.1 NT-GFP
pCMV-Tag 4B
pF5K CMV-neo
pTet-On-Advanced
pcDNA3.1 nV5-DEST
pCMV-Tag 4C
pFC14A HaloTag CMV
pTet-tTS
pcDNA3.1 V5-His A
pCMV-Tag 5A
pFC14K HaloTag CMV
pTetOne
pcDNA3.1 V5-His B
pCMV-Tag 5B
pFC15A HaloTag CMVd1
pTK-neo
pcDNA3.1 V5-His C
pCMV-Tag 5C
pFC15K HaloTag CMVd1
pTracer-CMV
pcDNA3.1 Zeo(+)
pCMV-Tet3G
pFC16A HaloTag CMVd2
pTracer-CMV Bsd
pcDNA3.1 Zeo(-)
pCMV6-A-BSD
pFC16K HaloTag CMVd2
pTracer-CMV2
pcDNA3.2 V5-DEST
pCMV6-A-EM7-BSD
pFC17A HaloTag CMVd3
pTracer-EF V5-His A
pcDNA4 His A
pCMV6-A-GFP
pFC17K HaloTag CMVd3
pTracer-EF V5-His B
pcDNA4 His B
pCMV6-A-Hygro
pFC27A HaloTag CMV-neo
pTracer-EF V5-His C
pcDNA4 His C
pCMV6-A-Puro
pFC27K HaloTag CMV-neo
pTracer-SV40
pcDNA4 HisMax A
pCMV6-AC
pFLAG-CMV-1
pTRE-Cycle1
pcDNA4 HisMax B
pCMV6-AC-3DDK
pFLAG-CMV-2
pTRE-Cycle2
pcDNA4 HisMax C
pCMV6-AC-3HA
pFLAG-CMV-3
pTRE-Cycle3
pcDNA4 myc-His A
pCMV6-AC-DDK
pFLAG-CMV-4
pTRE-Dual1
pcDNA4 myc-His B
pCMV6-AC-DDK-His
pFLAG-CMV-5.1
pTRE-Dual2
pcDNA4 myc-His C
pCMV6-AC-FC
pFLAG-Myc-CMV-19
pTRE-Tight
pcDNA4 TO
pCMV6-AC-FC-S
pFLAG-Myc-CMV-20
pTRE-Tight-BI
pcDNA4 TO myc-His A
pCMV6-AC-FP602
pFLAG-Myc-CMV-21
pTRE-Tight-BI-AcGFP1
pcDNA4 TO myc-His B
pCMV6-AC-FP635
pFLAG-Myc-CMV-22
pTRE-Tight-BI-DsRed-Express
pcDNA4 TO myc-His C
pCMV6-AC-GFP
pFN21A HaloTag CMV
pTRE-Tight-BI-ZsGreen1
pcDNA4 V5-His A
pCMV6-AC-GFP-rtTA
pFN21K HaloTag CMV
pTRE2
pcDNA4 V5-His B
pCMV6-AC-HA
pFN22A HaloTag CMVd1
pTRE2hyg
pcDNA4 V5-His C
pCMV6-AC-HA-His
pFN22K HaloTag CMVd1
pTRE2pur
pcDNA5 FRT
pCMV6-AC-His
pFN23A HaloTag CMVd2
pTRE3G
pcDNA5 FRT TO
pCMV6-AC-IRES-GFP
pFN23K HaloTag CMVd2
pTRE3G-BI
pcDNA5 TO
pCMV6-AC-IRES-GFP-Puro
pFN24A HaloTag CMVd3
pTRE3G-BI-Het1
pcDNA6.2 C-EmGFP-DEST
pCMV6-AC-mBFP
pFN24K HaloTag CMVd3
pTRE3G-BI-mCherry
pcDNA6.2 C-YFP-DEST
pCMV6-AC-mCFP
pFN28A HaloTag CMV-neo
pTRE3G-BI-ZsGreen1
pcDNA6.2 cGeneBLAzer-DEST
pCMV6-AC-mGFP
pFN28K HaloTag CMV-neo
pTRE3G-IRES
pcDNA6.2 cLumio-DEST
pCMV6-AC-mKate
pFRT lacZeo
pTRE3G-mCherry
pcDNA6.2 EmGFP-Bsd V5-DEST
pCMV6-AC-mRFP
pFRT lacZeo2
pTRE3G-ZsGreen1
pcDNA6.2 N-EmGFP-DEST
pCMV6-AC-Myc
pGen2.1
pVAX1
pcDNA6.2 N-YFP-DEST
pCMV6-AC-Myc-DDK
phCMV1
pX330
pcDNA6.2 nGeneBLAzer-DEST
pCMV6-AC-Myc-His
phCMV2
pZeoSV2(+)
pcDNA6.2 nLumio-DEST
pCMV6-AC-mYFP
phCMV3
pZeoSV2(-)
pcDNA6.2 V5-DEST
pCMV6-AC-RFP
pHet-1
pZFHD1-2
pcDNA6.2 V5-pL-DEST

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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