pCMV-Tag 5C
Mammalian expression vector for tagging proteins with a C-terminal Myc epitope. For other reading frames, use pCMV-Tag 5A or pCMV-Tag 5B.
Sequence Author: Agilent Technologies
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BsrDI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different BstXI sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present. Sticky ends from different EarI sites may not be compatible. |
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ApaI can be used between 25°C and 37°C. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different BtsαI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
| NeoR/KanR 2226 .. 3020 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
| NeoR/KanR 2226 .. 3020 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
| ori 3628 .. 4216 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3628 .. 4216 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1246 .. 1701 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1246 .. 1701 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| SV40 promoter 1834 .. 2191 = 358 bp SV40 enhancer and early promoter |
| SV40 promoter 1834 .. 2191 = 358 bp SV40 enhancer and early promoter |
| CMV enhancer 66 .. 370 = 305 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 66 .. 370 = 305 bp human cytomegalovirus immediate early enhancer |
| CMV promoter 371 .. 574 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 371 .. 574 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 1118 .. 1239 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 1118 .. 1239 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 1728 .. 1830 = 103 bp |
| AmpR promoter 1728 .. 1830 = 103 bp |
| MCS 651 .. 745 = 95 bp multiple cloning site |
| MCS 651 .. 745 = 95 bp multiple cloning site |
| HSV TK poly(A) signal 3252 .. 3299 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
| HSV TK poly(A) signal 3252 .. 3299 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
| T3 promoter 620 .. 638 = 19 bp promoter for bacteriophage T3 RNA polymerase |
| T3 promoter 620 .. 638 = 19 bp promoter for bacteriophage T3 RNA polymerase |
| T7 promoter 826 .. 844 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| T7 promoter 826 .. 844 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| stop codons 788 .. 798 = 11 bp stop codons in all three reading frames |
| stop codons 788 .. 798 = 11 bp stop codons in all three reading frames |
| Myc 743 .. 772 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
| Myc 743 .. 772 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
| ORF: 2398 .. 2784 = 387 bp ORF: 128 amino acids = 14.6 kDa |
| ORF: 3041 .. 3490 = 450 bp ORF: 149 amino acids = 16.3 kDa |
| ORF: 2226 .. 3020 = 795 bp ORF: 264 amino acids = 29.0 kDa |
| ORF: 2535 .. 3071 = 537 bp ORF: 178 amino acids = 19.9 kDa |
| ORF: 3246 .. 3479 = 234 bp ORF: 77 amino acids = 8.6 kDa |
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Download pCMV-Tag 5C.dna file
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