pCMV6-AC-mYFP
PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal TagYFP tag.
Sequence Author: OriGene
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsmI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different PasI sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different BstXI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
VP1.5 (forward primer) 18-mer / 44% GC 1 binding site 839 .. 856 = 18 annealed bases Tm = 51°C |
XL39 (reverse primer) 20-mer / 55% GC 1 binding site 1929 .. 1948 = 20 annealed bases Tm = 59°C |
AmpR 5212 .. 6072 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 5212 .. 6003 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 5212 .. 6072 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 6004 .. 6072 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 5212 .. 6072 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
NeoR/KanR 2913 .. 3707 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
NeoR/KanR 2913 .. 3707 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
TagYFP 1088 .. 1804 = 717 bp 239 amino acids = 27.0 kDa Product: monomeric yellow fluorescent protein mammalian codon optimized |
TagYFP 1088 .. 1804 = 717 bp 239 amino acids = 27.0 kDa Product: monomeric yellow fluorescent protein mammalian codon optimized |
hGH poly(A) signal 1866 .. 2488 = 623 bp human growth hormone polyadenylation signal |
hGH poly(A) signal 1866 .. 2488 = 623 bp human growth hormone polyadenylation signal |
ori 4453 .. 5041 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4453 .. 5041 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 6204 .. 25 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 6204 .. 25 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
CMV enhancer 343 .. 722 = 380 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 343 .. 722 = 380 bp human cytomegalovirus immediate early enhancer |
SV40 promoter 2517 .. 2846 = 330 bp SV40 enhancer and early promoter |
SV40 promoter 2517 .. 2846 = 330 bp SV40 enhancer and early promoter |
CMV promoter 723 .. 926 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 723 .. 926 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 3881 .. 4002 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 3881 .. 4002 = 122 bp SV40 polyadenylation signal |
MCS 979 .. 1087 = 109 bp multiple cloning sequence |
MCS 979 .. 1087 = 109 bp multiple cloning sequence |
AmpR promoter 6073 .. 6177 = 105 bp |
AmpR promoter 6073 .. 6177 = 105 bp |
T7 promoter 952 .. 970 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 952 .. 970 = 19 bp promoter for bacteriophage T7 RNA polymerase |
SV40 ori 2697 .. 2832 = 136 bp SV40 origin of replication |
SV40 ori 2697 .. 2832 = 136 bp SV40 origin of replication |
ORF: 3085 .. 3471 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 1088 .. 1819 = 732 bp ORF: 243 amino acids = 27.4 kDa |
ORF: 5342 .. 5608 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 2913 .. 3707 = 795 bp ORF: 264 amino acids = 29.0 kDa |
ORF: 1052 .. 1966 = 915 bp ORF: 304 amino acids = 30.6 kDa |
ORF: 2230 .. 2454 = 225 bp ORF: 74 amino acids = 8.1 kDa |
ORF: 5212 .. 6072 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 3222 .. 3758 = 537 bp ORF: 178 amino acids = 19.7 kDa |
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