pcDNA6 V5-His C
Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA™6/V5-His A or pcDNA™6/V5-His B.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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| * Blocked by Dam methylation. |
| AmpR 4144 .. 5004 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4144 .. 4935 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4144 .. 5004 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4936 .. 5004 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4144 .. 5004 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 3385 .. 3973 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3385 .. 3973 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1372 .. 1800 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1372 .. 1800 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| BSD 2257 .. 2655 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
| BSD 2257 .. 2655 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| SV40 promoter 1814 .. 2143 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 1814 .. 2143 = 330 bp SV40 enhancer and early promoter |
| bGH poly(A) signal 1102 .. 1326 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 1102 .. 1326 = 225 bp bovine growth hormone polyadenylation signal |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 2813 .. 2934 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 2813 .. 2934 = 122 bp SV40 polyadenylation signal |
| MCS 895 .. 1004 = 110 bp multiple cloning site |
| MCS 895 .. 1004 = 110 bp multiple cloning site |
| AmpR promoter 5005 .. 5109 = 105 bp |