pCMV6-AN-Myc-DDK

PrecisionShuttle™ mammalian expression vector for fusing an ORF to N-terminal c-Myc and FLAG® tags.

Sequence Author: OriGene

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
BsrGI (301) AanI (5887) DraIII (5762) XmnI (5209) ScaI (5090) AlwNI (4136) PciI (3720) BstZ17I (3341) BsmI (3289) AanI (3228) PfoI (3144) BstBI (3051) PflFI - Tth111I (2487) NdeI (592) SnaBI (698) CMV promoter VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) ATG Myc FLAG AsiSI - SgfI (1081) MreI - SgrAI (1083) AscI (1087) HindIII (1101) RsrII (1118) MluI (1124) NotI (1134) PaeR7I - PspXI - XhoI (1142) PmeI (1151) FseI (1161) SacII (1165) AleI (1251) XL39 (reverse primer) (1257 .. 1276) BbsI (1374) EcoO109I (1381) BsgI (1435) BbvCI - Bpu10I (1477) BlpI (1521) BsmBI (1541) BstXI (1628) AgeI (1739) PflMI (1742) XcmI (1834) SexAI * (1926) SfiI (2112) StuI (2158) AvrII (2159) BclI * (2210) BsaBI * (2228) KasI (2368) NarI (2369) SfoI (2370) PluTI (2372) PstI (2422) pCMV6-AN-Myc-DDK 5959 bp
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AanI  (5887)
2 sites
T T A T A A A A T A T T
DraIII  (5762)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (5209)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5090)
1 site
A G T A C T T C A T G A
AlwNI  (4136)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3720)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3341)
1 site
G T A T A C C A T A T G
BsmI  (3289)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3228)
2 sites
T T A T A A A A T A T T
PfoI  (3144)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3051)
1 site
T T C G A A A A G C T T
PflFI  (2487)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2487)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
AsiSI  (1081)
1 site
G C G A T C G C C G C T A G C G
SgfI  (1081)
1 site
G C G A T C G C C G C T A G C G
MreI  (1083)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (1083)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1087)
1 site
G G C G C G C C C C G C G C G G
HindIII  (1101)
1 site
A A G C T T T T C G A A
RsrII  (1118)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (1124)
1 site
A C G C G T T G C G C A
NotI  (1134)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1142)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1142)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1142)
1 site
C T C G A G G A G C T C
PmeI  (1151)
1 site
G T T T A A A C C A A A T T T G
FseI  (1161)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SacII  (1165)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (1251)
1 site
C A C N N N N G T G G T G N N N N C A C
BbsI  (1374)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (1381)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsgI  (1435)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbvCI  (1477)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1477)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BlpI  (1521)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsmBI  (1541)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BstXI  (1628)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AgeI  (1739)
1 site
A C C G G T T G G C C A
PflMI  (1742)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
XcmI  (1834)
1 site
C C A N N N N N N N N N T G G G