pSecTag B
Mammalian expression vector for the secretion of C-terminally 6xHis-tagged proteins (replaced by pSecTag2 B). For other reading frames, use pSecTag A or pSecTag C.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
| AmpR 4174 .. 5034 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4174 .. 4965 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4174 .. 5034 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4966 .. 5034 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4174 .. 5034 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 3415 .. 4003 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3415 .. 4003 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1454 .. 1882 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1454 .. 1882 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| BleoR 2339 .. 2713 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| BleoR 2339 .. 2713 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| SV40 promoter 1896 .. 2225 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 1896 .. 2225 = 330 bp SV40 enhancer and early promoter |
| bGH poly(A) signal 1184 .. 1408 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 1184 .. 1408 = 225 bp bovine growth hormone polyadenylation signal |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| MCS 970 .. 1092 = 123 bp multiple cloning site |
| MCS 970 .. 1092 = 123 bp multiple cloning site |
| SV40 poly(A) signal 2843 .. 2964 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 2843 .. 2964 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 5035 .. 5139 = 105 bp |
| AmpR promoter 5035 .. 5139 = 105 bp |
| Igκ leader 905 .. 967 = 63 bp 21 amino acids = 2.4 kDa Product: leader sequence from mouse immunoglobulin κ light chain |
| Igκ leader 905 .. 967 = 63 bp 21 amino acids = 2.4 kDa Product: leader sequence from mouse immunoglobulin κ light chain |
| Myc 1093 .. 1122 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
| Myc 1093 .. 1122 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
| 6xHis 1138 .. 1155 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 1138 .. 1155 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| EM7 promoter 2273 .. 2320 = 48 bp synthetic bacterial promoter |
| EM7 promoter 2273 .. 2320 = 48 bp synthetic bacterial promoter |
| T7 promoter 863 .. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| T7 promoter 863 .. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| SV40 ori 2076 .. 2211 = 136 bp SV40 origin of replication |
| SV40 ori 2076 .. 2211 = 136 bp SV40 origin of replication |
| ORF: 1405 .. 1671 = 267 bp ORF: 88 amino acids = 9.3 kDa |
| ORF: 2339 .. 2713 = 375 bp ORF: 124 amino acids = 13.8 kDa |
| ORF: 4304 .. 4570 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 2332 .. 2619 = 288 bp ORF: 95 amino acids = 10.1 kDa |
| ORF: 4174 .. 5034 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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