pTet-DualON

Vector for co-expressing ZsGreen1 with the Tet-On® Advanced transcriptional activator.

Sequence Author: Clontech (TaKaRa)

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SspI (4719) EarI (4710) ScaI (4395) PvuI (4285) FspI (4137) AseI (4087) BsaI (3976) AlwNI (3438) DrdI (3130) PsiI (2839) HpaI (2819) BsaBI * (2718) EcoRV (2703) BspDI - ClaI (2684) NotI (2676) Bpu10I (2671) BsrGI (2529) PaeR7I - PspXI - XhoI (1) SnaBI (289) Eco53kI (515) SacI (517) BsmBI - Esp3I (538) SacII (604) BamHI (607) PvuII (621) MluI (625) StuI (704) NruI (914) FseI (1258) TspMI - XmaI (1372) SmaI (1374) EcoRI (1384) AvrII (1543) PaqCI (1731) DraIII (1755) Acc65I (1833) KpnI (1837) PflMI (1845) MscI (1970) BstXI (1978) BclI * (2061) SgrAI (2066) PflFI - Tth111I (2213) KasI (2282) NarI (2283) SfoI (2284) PluTI (2286) pTet-DualON 4906 bp
SspI  (4719)
1 site
A A T A T T T T A T A A
EarI  (4710)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
ScaI  (4395)
1 site
A G T A C T T C A T G A
PvuI  (4285)
1 site
C G A T C G G C T A G C
FspI  (4137)
1 site
T G C G C A A C G C G T
AseI  (4087)
1 site
A T T A A T T A A T T A
BsaI  (3976)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AlwNI  (3438)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
DrdI  (3130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PsiI  (2839)
1 site
T T A T A A A A T A T T
HpaI  (2819)
1 site
G T T A A C C A A T T G
BsaBI  (2718)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
EcoRV  (2703)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BspDI  (2684)
1 site
A T C G A T T A G C T A