Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
ScaI (4395) 1 site
AGTACTTCATGA
PvuI (4285) 1 site
CGATCGGCTAGC
FspI (4137) 1 site
TGCGCAACGCGT
AseI (4087) 1 site
ATTAATTAATTA
BsaI (3976) 1 site
GGTCTCNCCAGAGN(N)4
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C.
AlwNI (3438) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
DrdI (3130) 1 site
GACNNNNNNGTCCTGNNNNNNCAG
Sticky ends from different DrdI sites may not be compatible.