Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.
SspI (3752) 1 site
ScaI (3428) 1 site
PvuI (3318) 1 site
FspI (3170) 1 site
NmeAIII (3096) 1 site
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
BpmI (3018) 1 site
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
AhdI (2948) 1 site
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
PspFI (2521) 1 site
BseYI (2517) 1 site
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
DrdI (2321) 1 site
Sticky ends from different DrdI sites may not be compatible.
