pTRE-Dual1
Vector for co-expressing two genes with the Tet-On® Advanced or Tet-Off® Advanced system.
Sequence Author: Clontech (TaKaRa)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
|
|
| ||
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present. Sticky ends from different EarI sites may not be compatible. |
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
| ||
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
Sticky ends from different AlwNI sites may not be compatible. |
|
|
|
|
|
|
|
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
|
|
|
|
|
| ||
ApaI can be used between 25°C and 37°C. |
|
| ||
Sticky ends from different StyI sites may not be compatible. |
|
|
|
| ||
Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
| ||
Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
| ||
Sticky ends from different DraIII sites may not be compatible. |
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
|
|
| ||
Sticky ends from different PflMI sites may not be compatible. |
| ||
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
|
|
|