The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
PciI (5496) 1 site
PciI is inhibited by nonionic detergents.
BspQI (5380) 1 site
Sticky ends from different BspQI sites may not be compatible.
SapI (5380) 1 site
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PpuMI (4588) 1 site
Sticky ends from different PpuMI sites may not be compatible.
BbsI (4514) 1 site
Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C.
BclI (4351) 1 site
* Blocked by Dam methylation.BclI is typically used at 50-55°C, but is 50% active at 37°C.
AvrII (4300) 1 site
StuI (4299) 1 site
CsiI (4067) 1 site
Sticky ends from different CsiI sites may not be compatible.
SexAI (4067) 1 site
* Blocked by Dcm methylation.Sticky ends from different SexAI sites may not be compatible.
