pCMV6-AN-mCFP
PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal TagCFP tag.
Sequence Author: OriGene
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Sticky ends from different AlwNI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsmI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different PasI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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FseI gradually loses activity when stored at -20°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different BstXI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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| VP1.5 (forward primer) 18-mer / 44% GC 1 binding site 839 .. 856 = 18 annealed bases Tm = 51°C |
| XL39 (reverse primer) 20-mer / 55% GC 1 binding site 1926 .. 1945 = 20 annealed bases Tm = 59°C |
| AmpR 5209 .. 6069 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 5209 .. 6000 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5209 .. 6069 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 6001 .. 6069 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5209 .. 6069 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| NeoR/KanR 2910 .. 3704 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
| NeoR/KanR 2910 .. 3704 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
| TagCFP 1020 .. 1733 = 714 bp 238 amino acids = 26.7 kDa Product: monomeric cyan fluorescent protein mammalian codon-optimized |
| TagCFP 1020 .. 1733 = 714 bp 238 amino acids = 26.7 kDa Product: monomeric cyan fluorescent protein mammalian codon-optimized |
| hGH poly(A) signal 1863 .. 2485 = 623 bp human growth hormone polyadenylation signal |
| hGH poly(A) signal 1863 .. 2485 = 623 bp human growth hormone polyadenylation signal |
| ori 4450 .. 5038 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 4450 .. 5038 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 6201 .. 25 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 6201 .. 25 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 343 .. 722 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 343 .. 722 = 380 bp human cytomegalovirus immediate early enhancer |
| SV40 promoter 2514 .. 2843 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 2514 .. 2843 = 330 bp SV40 enhancer and early promoter |
| CMV promoter 723 .. 926 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 723 .. 926 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 3878 .. 3999 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 3878 .. 3999 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 6070 .. 6174 = 105 bp |
| AmpR promoter 6070 .. 6174 = 105 bp |
| MCS 1734 .. 1832 = 99 bp multiple cloning site |
| MCS 1734 .. 1832 = 99 bp multiple cloning site |
| T7 promoter 952 .. 970 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| T7 promoter 952 .. 970 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| SV40 ori 2694 .. 2829 = 136 bp SV40 origin of replication |
| SV40 ori 2694 .. 2829 = 136 bp SV40 origin of replication |
| ORF: 3082 .. 3468 = 387 bp ORF: 128 amino acids = 14.6 kDa |
| ORF: 5339 .. 5605 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 882 .. 1781 = 900 bp ORF: 299 amino acids = 33.6 kDa |
| ORF: 2910 .. 3704 = 795 bp ORF: 264 amino acids = 29.0 kDa |
| ORF: 1292 .. 1531 = 240 bp ORF: 79 amino acids = 9.3 kDa |