pcDNA4 TO myc-His A
Vector for tetracycline-inducible expression of C-terminally Myc- and 6xHis-tagged proteins. For other reading frames, use pcDNA™4/TO/myc-His B or pcDNA™4/TO/myc-His C.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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ApaI can be used between 25°C and 37°C. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
| AmpR 4155 .. 5015 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4155 .. 4946 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4155 .. 5015 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4947 .. 5015 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4155 .. 5015 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 3396 .. 3984 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3396 .. 3984 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1434 .. 1862 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1434 .. 1862 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| BleoR 2320 .. 2694 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| BleoR 2320 .. 2694 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| SV40 promoter 1876 .. 2206 = 331 bp SV40 enhancer and early promoter |
| SV40 promoter 1876 .. 2206 = 331 bp SV40 enhancer and early promoter |
| bGH poly(A) signal 1164 .. 1388 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 1164 .. 1388 = 225 bp bovine growth hormone polyadenylation signal |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 2824 .. 2945 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 2824 .. 2945 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 5016 .. 5120 = 105 bp |
| AmpR promoter 5016 .. 5120 = 105 bp |
| MCS 968 .. 1069 = 102 bp multiple cloning site |
| MCS 968 .. 1069 = 102 bp multiple cloning site |
| Myc 1073 .. 1102 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
| Myc 1073 .. 1102 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
| 6xHis 1118 .. 1135 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 1118 .. 1135 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| EM7 promoter 2254 .. 2301 = 48 bp synthetic bacterial promoter |
| EM7 promoter 2254 .. 2301 = 48 bp synthetic bacterial promoter |
| tet operator 820 .. 838 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 820 .. 838 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 841 .. 859 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 841 .. 859 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| SV40 ori 2057 .. 2192 = 136 bp SV40 origin of replication |
| SV40 ori 2057 .. 2192 = 136 bp SV40 origin of replication |
| TATA box 804 .. 810 = 7 bp |
| TATA box 804 .. 810 = 7 bp |
| ORF: 2320 .. 2694 = 375 bp ORF: 124 amino acids = 13.8 kDa |
| ORF: 4285 .. 4551 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 1385 .. 1651 = 267 bp ORF: 88 amino acids = 9.3 kDa |
| ORF: 774 .. 1103 = 330 bp ORF: 109 amino acids = 12.8 kDa |
| ORF: 2313 .. 2600 = 288 bp ORF: 95 amino acids = 10.1 kDa |
| ORF: 4155 .. 5015 = 861 bp |