pIRES

IRES-containing vector for expressing two genes in mammalian cells from the same bicistronic transcript.

Sequence Author: Clontech (TaKaRa)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
HomePlasmidsMammalian Expression VectorspIRES
BglII (6100) AlwNI (5679) AhdI (5200) BpmI (5131) PfoI (4161) BstXI (3945) BstBI (3906) RsrII (3740) BssHII (3621) PflFI - Tth111I (3342) PluTI (3227) SfoI (3225) NarI (3224) KasI (3223) BsrGI (96) SpeI (152) CMV enhancer NdeI (387) SnaBI (493) AsiSI (664) Eco53kI (727) SacI (729) I-PpoI (851) NheI (1085) BmtI (1089) PaeR7I - XhoI (1091) MCS A EcoRI (1096) MluI (1102) PspOMI (1265) ApaI (1269) PmlI (1468) PaqCI (1491) PflMI (1605) PciI (1642) BmgBI (1695) XbaI (1747) SalI (1753) AccI (1754) NotI (1764) T3 promoter HpaI (1930) MfeI (1939) CsiI - SexAI * (2797) SfiI (2983) StuI (3029) pIRES 6105 bp
BglII  (6100)
1 site		 A G A T C T T C T A G A
AlwNI  (5679)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AhdI  (5200)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (5131)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PfoI  (4161)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BstXI  (3945)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BstBI  (3906)
1 site		 T T C G A A A A G C T T
RsrII  (3740)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (3621)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (3342)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3342)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (3227)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3225)
1 site		 G G C G C C C C G C G G
NarI  (3224)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3223)
1 site		 G G C G C C C C G C G G
BsrGI  (96)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (152)
1 site		 A C T A G T T G A T C A
NdeI  (387)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (493)
1 site		 T A C G T A A T G C A T
AsiSI  (664)
1 site		 G C G A T C G C C G C T A G C G
Eco53kI  (727)
1 site		 G A G C T C C T C G A G
SacI  (729)
1 site		 G A G C T C C T C G A G
I-PpoI  (851)
1 site		 C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G
I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences.
NheI  (1085)
1 site		 G C T A G C C G A T C G
BmtI  (1089)
1 site		 G C T A G C C G A T C G
PaeR7I  (1091)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1091)
1 site		 C T C G A G G A G C T C
EcoRI  (1096)
1 site		 G A A T T C C T T A A G
MluI  (1102)
1 site		 A C G C G T T G C G C A
PspOMI  (1265)
1 site		 G G G C C C C C C G G G
ApaI  (1269)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PmlI  (1468)
1 site		 C A C G T G G T G C A C
PaqCI  (1491)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
PflMI  (1605)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
PciI  (1642)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BmgBI  (1695)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
XbaI  (1747)
1 site		 T C T A G A A G A T C T
SalI  (1753)
1 site		 G T C G A C C A G C T G
AccI  (1754)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NotI  (1764)
1 site		 G C G G C C G C C G C C G G C G
HpaI  (1930)
1 site		 G T T A A C C A A T T G
MfeI  (1939)
1 site		 C A A T T G G T T A A C
CsiI  (2797)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2797)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (2983)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (3029)
1 site		 A G G C C T T C C G G A
AmpR
4413 .. 5273  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4413 .. 4481  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4413 .. 5273  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4482 .. 5273  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4413 .. 5273  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
3096 .. 3890  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
3096 .. 3890  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
ori
5444 .. 6032  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5444 .. 6032  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
1152 .. 1725  =  574 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
1152 .. 1725  =  574 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
f1 ori
2116 .. 2571  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2116 .. 2571  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2688 .. 3045  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2688 .. 3045  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
213 .. 517  =  305 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
213 .. 517  =  305 bp
human cytomegalovirus immediate early enhancer
chimeric intron
890 .. 1022  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
chimeric intron
890 .. 1022  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
SV40 poly(A) signal
1809 .. 1930  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1809 .. 1930  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4308 .. 4412  =  105 bp
AmpR promoter
4308 .. 4412  =  105 bp
poly(A) signal
3954 .. 4002  =  49 bp
synthetic polyadenylation signal
poly(A) signal
3954 .. 4002  =  49 bp
synthetic polyadenylation signal
minimal CMV promoter
691 .. 729  =  39 bp
human cytomegalovirus (CMV) immediate early promoter
minimal CMV promoter
691 .. 729  =  39 bp
human cytomegalovirus (CMV) immediate early promoter
MCS B
1737 .. 1770  =  34 bp
multiple cloning site
MCS B
1737 .. 1770  =  34 bp
multiple cloning site
MCS A
1085 .. 1107  =  23 bp
multiple cloning site
MCS A
1085 .. 1107  =  23 bp
multiple cloning site
T3 promoter
1774 .. 1791  =  18 bp
promoter for bacteriophage T3 RNA polymerase (shorter by one base than the standard T3 promoter)
T3 promoter
1774 .. 1791  =  18 bp
promoter for bacteriophage T3 RNA polymerase (shorter by one base than the standard T3 promoter)
SV40 ori
2896 .. 3031  =  136 bp
SV40 origin of replication
SV40 ori
2896 .. 3031  =  136 bp
SV40 origin of replication
T7 promoter
1067 .. 1085  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1067 .. 1085  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  3268 .. 3654  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  3096 .. 3890  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  4413 .. 5273  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3405 .. 3941  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  4877 .. 5143  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pIRES.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

p3xFLAG-CMV-10
pcDNA6 myc-His A
pCMV6-AC-YFP
pHet-Act1-2
p3xFLAG-CMV-13
pcDNA6 myc-His B
pCMV6-AN-3DDK
pHet-Mem1
p3xFLAG-CMV-14
pcDNA6 myc-His C
pCMV6-AN-3HA
pHet-Nuc1
p3xFLAG-CMV-7