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pME-HA (linearized)
Linearized mammalian cell expression vector for directional recombination-based cloning of a gene in the Expresso® system.
Sequence Author: Lucigen
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Mammalian Expression Vectors
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Plasmids
Mammalian Expression Vectors
pME-HA (linearized)
Show Static Map
Eco53kI
(3324)
CMV promoter
SnaBI
(3098)
ApaI
(2755)
PspOMI
(2751)
ApaLI
(2439)
XcmI
(2034)
BsaI
(1911)
PfoI
(1697)
BstBI
(1604)
SacI
(3326)
pME Forward
(3344 .. 3365)
BtsI
-
BtsαI
(3346)
NheI
(3382)
BmtI
(3386)
End
(3416)
Start
(0)
BmgBI
(12)
AvaI
-
BsoBI
-
PaeR7I
-
XhoI
(38)
pME Reverse
(59 .. 80)
PsiI
(208)
ApoI
(263)
AseI
(277)
XmnI
(278)
BspHI
(348)
SspI
(383)
Bsu36I
(402)
CsiI
-
SexAI
*
(511)
SfiI
(697)
BseRI
(740)
StuI
(743)
BsaBI
*
(781)
EagI
(828)
KasI
(921)
NarI
(922)
SfoI
(923)
PluTI
(925)
DrdI
(949)
MscI
(1004)
FspI
(1024)
PflFI
-
Tth111I
(1040)
BsrDI
(1155)
BtgI
(1354)
RsrII
(1438)
Start
(0)
Start
(0)
Start
BmgBI
(12)
BmgBI
(12)
BmgBI
XhoI
(38)
XhoI
(38)
XhoI
AvaI
-
(38)
AvaI
-
(38)
AvaI
-
BsoBI
-
(38)
BsoBI
-
(38)
BsoBI
-
PaeR7I
-
(38)
PaeR7I
-
(38)
PaeR7I
-
PsiI
(208)
PsiI
(208)
PsiI
ApoI
(263)
ApoI
(263)
ApoI
AseI
(277)
AseI
(277)
AseI
XmnI
(278)
XmnI
(278)
XmnI
BspHI
(348)
BspHI
(348)
BspHI
SspI
(383)
SspI
(383)
SspI
Bsu36I
(402)
Bsu36I
(402)
Bsu36I
SexAI
*
(511)
SexAI
*
(511)
SexAI
*
CsiI
-
(511)
CsiI
-
(511)
CsiI
-
SfiI
(697)
SfiI
(697)
SfiI
BseRI
(740)
BseRI
(740)
BseRI
StuI
(743)
StuI
(743)
StuI
BsaBI
*
(781)
BsaBI
*
(781)
BsaBI
*
EagI
(828)
EagI
(828)
EagI
KasI
(921)
KasI
(921)
KasI
NarI
(922)
NarI
(922)
NarI
SfoI
(923)
SfoI
(923)
SfoI
PluTI
(925)
PluTI
(925)
PluTI
DrdI
(949)
DrdI
(949)
DrdI
MscI
(1004)
MscI
(1004)
MscI
FspI
(1024)
FspI
(1024)
FspI
Tth111I
(1040)
Tth111I
(1040)
Tth111I
PflFI
-
(1040)
PflFI
-
(1040)
PflFI
-
BsrDI
(1155)
BsrDI
(1155)
BsrDI
BtgI
(1354)
BtgI
(1354)
BtgI
RsrII
(1438)
RsrII
(1438)
RsrII
BstBI
(1604)
BstBI
(1604)
BstBI
PfoI
(1697)
PfoI
(1697)
PfoI
BsaI
(1911)
BsaI
(1911)
BsaI
XcmI
(2034)
XcmI
(2034)
XcmI
ApaLI
(2439)
ApaLI
(2439)
ApaLI
PspOMI
(2751)
PspOMI
(2751)
PspOMI
ApaI
(2755)
ApaI
(2755)
ApaI
SnaBI
(3098)
SnaBI
(3098)
SnaBI
Eco53kI
(3324)
Eco53kI
(3324)
Eco53kI
SacI
(3326)
SacI
(3326)
SacI
BtsI
-
(3346)
BtsI
-
(3346)
BtsI
-
BtsαI
(3346)
BtsαI
(3346)
BtsαI
NheI
(3382)
NheI
(3382)
NheI
BmtI
(3386)
BmtI
(3386)
BmtI
End
(3416)
End
(3416)
End
pME Forward
(3344 .. 3365)
pME Forward
pME Forward
(3344 .. 3365)
pME Reverse
(59 .. 80)
pME Reverse
pME Reverse
(59 .. 80)
CMV promoter
CMV promoter
CMV promoter
CMV promoter
pME-HA
3416 bp
Eco53kI (3324)
1 site
G
A
G
C
T
C
C
T
C
G
A
G
SnaBI (3098)
1 site
T
A
C
G
T
A
A
T
G
C
A
T
ApaI (2755)
1 site
G
G
G
C
C
C
C
C
C
G
G
G
ApaI can be used between 25°C and 37°C.
PspOMI (2751)
1 site
G
G
G
C
C
C
C
C
C
G
G
G
ApaLI (2439)
1 site
G
T
G
C
A
C
C
A
C
G
T
G
XcmI (2034)
1 site
C
C
A
N
N
N
N
N
N
N
N
N
T
G
G
G
G
T
N
N
N
N
N
N
N
N
N
A
C
C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsaI (1911)
1 site
G
G
T
C
T
C
N
C
C
A
G
A
G
N
(
N
)
4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI (1697)
1 site
T
C
C
N
G
G
A
A
G
G
N
C
C
T
Sticky ends from different PfoI sites may not be compatible.
BstBI (1604)
1 site
T
T
C
G
A
A
A
A
G
C
T
T
SacI (3326)
1 site
G
A
G
C
T
C
C
T
C
G
A
G
BtsI (3346)
1 site
G
C
A
G
T
G
N
N
C
G
T
C
A
C
BtsαI (3346)
1 site
G
C
A
G
T
G
N
N
C
G
T
C
A
C
Sticky ends from different BtsαI sites may not be compatible.
NheI (3382)
1 site
G
C
T
A
G
C
C
G
A
T
C
G
BmtI (3386)
1 site
