pTetOne
All-in-one vector for strong inducible expression of genes in mammalian cells using the Tet-On® system.
Sequence Author: Clontech (TaKaRa)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BglI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different StyI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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