pTetOne
All-in-one vector for strong inducible expression of genes in mammalian cells using the Tet-On® system.
Sequence Author: Clontech (TaKaRa)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BglI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different StyI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
| AmpR 2959 .. 3819 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 2959 .. 3750 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 2959 .. 3819 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 3751 .. 3819 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 2959 .. 3819 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| Tet-On® 3G 1074 .. 1820 = 747 bp 248 amino acids = 27.7 kDa Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline |
| Tet-On® 3G 1074 .. 1820 = 747 bp 248 amino acids = 27.7 kDa Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline |
| ori 2200 .. 2788 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 2200 .. 2788 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| hPGK promoter 545 .. 1055 = 511 bp human phosphoglycerate kinase 1 promoter |
| hPGK promoter 545 .. 1055 = 511 bp human phosphoglycerate kinase 1 promoter |
| TRE3GS promoter 1 .. 368 = 368 bp 3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors |
| TRE3GS promoter 1 .. 368 = 368 bp 3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors |
| AmpR promoter 3820 .. 3924 = 105 bp |
| AmpR promoter 3820 .. 3924 = 105 bp |
| pause site 372 .. 463 = 92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene |
| pause site 372 .. 463 = 92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene |
| SV40 poly(A) signal 1937 .. 2018 = 82 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 1937 .. 2018 = 82 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 3957 .. 4038 = 82 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 3957 .. 4038 = 82 bp SV40 polyadenylation signal |
| MCS 4158 .. 4207 = 50 bp multiple cloning site |
| MCS 4158 .. 4207 = 50 bp multiple cloning site |
| poly(A) signal 477 .. 525 = 49 bp synthetic polyadenylation signal |
| poly(A) signal 477 .. 525 = 49 bp synthetic polyadenylation signal |
| tet operator 126 .. 144 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 126 .. 144 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 162 .. 180 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 162 .. 180 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 198 .. 216 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 198 .. 216 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 234 .. 252 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 234 .. 252 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 270 .. 288 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 270 .. 288 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 306 .. 324 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 306 .. 324 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 342 .. 360 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 342 .. 360 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| ORF: 1231 .. 1533 = 303 bp ORF: 100 amino acids = 11.1 kDa |
| ORF: 3089 .. 3355 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 378 .. 779 = 402 bp ORF: 133 amino acids = 14.5 kDa |
| ORF: 837 .. 1820 = 984 bp ORF: 327 amino acids = 36.0 kDa |
| ORF: 68 .. 337 = 270 bp ORF: 89 amino acids = 10.5 kDa |
| ORF: 1526 .. 1846 = 321 bp ORF: 106 amino acids = 11.0 kDa |
| ORF: 412 .. 654 = 243 bp ORF: 80 amino acids = 9.0 kDa |
| ORF: 2959 .. 3819 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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