pTetOne

All-in-one vector for strong inducible expression of genes in mammalian cells using the Tet-On® system.

Sequence Author: Clontech (TaKaRa)

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NotI (4195) MluI (4188) AgeI (4176) BamHI (4170) BglII (4164) PstI (4162) AatII (3954) ZraI (3952) SspI (3836) XmnI (3631) PvuI (3402) AseI (3204) BglI (3152) AlwNI (2555) DrdI (2247) PciI (2139) EcoRI (4202) HindIII (114) BsgI (375) PaqCI (397) BstXI (548) StyI (566) PfoI (615) BsmBI - Esp3I - SpeI (801) MscI (867) BlpI (881) AscI (890) BspEI (995) StuI (1144) BsaAI - PmlI (1205) BseRI (1332) NruI (1354) TspMI - XmaI (1812) SmaI (1814) pTetOne™ 4207 bp
NotI  (4195)
1 site
G C G G C C G C C G C C G G C G
MluI  (4188)
1 site
A C G C G T T G C G C A
AgeI  (4176)
1 site
A C C G G T T G G C C A
BamHI  (4170)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BglII  (4164)
1 site
A G A T C T T C T A G A
PstI  (4162)
1 site
C T G C A G G A C G T C
AatII  (3954)
1 site
G A C G T C C T G C A G
ZraI  (3952)
1 site
G A C G T C C T G C A G
SspI  (3836)
1 site
A A T A T T T T A T A A
XmnI  (3631)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (3402)
1 site
C G A T C G G C T A G C
AseI  (3204)
1 site
A T T A A T T A A T T A
BglI  (3152)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AlwNI  (2555)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
DrdI  (2247)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PciI  (2139)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoRI  (4202)
1 site
G A A T T C C T T A A G
HindIII  (114)
1 site
A A G C T T T T C G A A
BsgI  (375)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PaqCI  (397)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BstXI  (548)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
StyI  (566)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
PfoI  (615)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BsmBI  (801)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (801)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
SpeI  (801)
1 site
A C T A G T T G A T C A
MscI  (867)
1 site
T G G C C A A C C G G T
BlpI  (881)
1 site
G C T