pEF6 V5-His C
Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5-His A or pEF6/V5-His B.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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| * Blocked by Dam methylation. |
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Sticky ends from different PpuMI sites may not be compatible. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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ApaI can be used between 25°C and 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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Sticky ends from different PasI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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| EF-1α promoter 473 .. 1651 = 1179 bp strong constitutive promoter for human elongation factor EF-1α |
| EF-1α promoter 473 .. 1651 = 1179 bp strong constitutive promoter for human elongation factor EF-1α |
| AmpR 4929 .. 5789 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4929 .. 5720 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4929 .. 5789 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 5721 .. 5789 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4929 .. 5789 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 4170 .. 4758 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 4170 .. 4758 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 2168 .. 2596 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 2168 .. 2596 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| BSD 3053 .. 3451 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
| BSD 3053 .. 3451 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
| SV40 promoter 2610 .. 2939 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 2610 .. 2939 = 330 bp SV40 enhancer and early promoter |
| bGH poly(A) signal 1898 .. 2122 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 1898 .. 2122 = 225 bp bovine growth hormone polyadenylation signal |
| SV40 poly(A) signal 3609 .. 3730 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 3609 .. 3730 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 5790 .. 91 = 105 bp |
| AmpR promoter 5790 .. 91 = 105 bp |
| MCS 1713 .. 1800 = 88 bp multiple cloning site |
| MCS 1713 .. 1800 = 88 bp multiple cloning site |
| V5 tag 1801 .. 1842 = 42 bp 14 amino acids = 1.4 kDa Product: epitope tag from simian virus 5 |
| V5 tag 1801 .. 1842 = 42 bp 14 amino acids = 1.4 kDa Product: epitope tag from simian virus 5 |
| 6xHis 1852 .. 1869 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 1852 .. 1869 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| EM7 promoter 2987 .. 3034 = 48 bp synthetic bacterial promoter |
| EM7 promoter 2987 .. 3034 = 48 bp synthetic bacterial promoter |
| T7 promoter 1668 .. 1686 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| T7 promoter 1668 .. 1686 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| EF-1α intron A 704 .. 1642 = 939 bp intron upstream of the start codon of human EF-1α |
| EF-1α intron A 704 .. 1642 = 939 bp intron upstream of the start codon of human EF-1α |
| SV40 ori 2790 .. 2925 = 136 bp SV40 origin of replication |
| SV40 ori 2790 .. 2925 = 136 bp SV40 origin of replication |
| ORF: 1108 .. 1341 = 234 bp ORF: 77 amino acids = 7.7 kDa |
| ORF: 2119 .. 2385 = 267 bp |