pTRE-Tight-BI-DsRed-Express
Vector for co-expressing DsRed-Express and another gene with the Tet-On® Advanced or Tet-Off® Advanced system.
Sequence Author: Clontech (TaKaRa)
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different PflMI sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present. Sticky ends from different EarI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different DrdI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BglI sites may not be compatible. |
AmpR 1541 .. 2401 = 861 bp 286 amino acids = 31.5 kDa 2 segments Segment 2: 1541 .. 2332 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 1541 .. 2401 = 861 bp 286 amino acids = 31.5 kDa 2 segments Segment 1: signal sequence 2333 .. 2401 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 1541 .. 2401 = 861 bp 286 amino acids = 31.5 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
DsRed-Express 2734 .. 3411 = 678 bp 225 amino acids = 25.7 kDa Product: rapidly maturing tetrameric variant of DsRed fluorescent protein mammalian codon-optimized |
DsRed-Express 2734 .. 3411 = 678 bp 225 amino acids = 25.7 kDa Product: rapidly maturing tetrameric variant of DsRed fluorescent protein mammalian codon-optimized |
ori 782 .. 1370 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 782 .. 1370 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
bidirectional TRE promoter 3422 .. 318 = 388 bp Tet-responsive bidirectional promoter PTight-BI, consisting of seven tet operator sequences flanked on each side by the minimal CMV promoter |
bidirectional TRE promoter 3422 .. 318 = 388 bp Tet-responsive bidirectional promoter PTight-BI, consisting of seven tet operator sequences flanked on each side by the minimal CMV promoter |
AmpR promoter 2402 .. 2506 = 105 bp |
AmpR promoter 2402 .. 2506 = 105 bp |
SV40 poly(A) signal 519 .. 600 = 82 bp SV40 polyadenylation signal |
SV40 poly(A) signal 519 .. 600 = 82 bp SV40 polyadenylation signal |
SV40 poly(A) signal 2539 .. 2620 = 82 bp SV40 polyadenylation signal |
SV40 poly(A) signal 2539 .. 2620 = 82 bp SV40 polyadenylation signal |
MCS 335 .. 405 = 71 bp multiple cloning site |
MCS 335 .. 405 = 71 bp multiple cloning site |
tet operator 12 .. 30 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 12 .. 30 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 48 .. 66 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 48 .. 66 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 83 .. 101 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 83 .. 101 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 119 .. 137 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 119 .. 137 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 155 .. 173 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 155 .. 173 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 190 .. 208 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 190 .. 208 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 226 .. 244 = 19 bp bacterial operator O2 for the tetR and tetA genes |
tet operator 226 .. 244 = 19 bp bacterial operator O2 for the tetR and tetA genes |
ORF: 70 .. 354 = 285 bp ORF: 94 amino acids = 11.0 kDa |
ORF: 1671 .. 1937 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 161 .. 418 = 258 bp ORF: 85 amino acids = 9.8 kDa |
ORF: 1541 .. 2401 = 861 bp ORF: 286 amino acids = 31.5 kDa |
ORF: 2729 .. 3490 = 762 bp ORF: 253 amino acids = 28.5 kDa |
ORF: 2734 .. 3411 = 678 bp ORF: 225 amino acids = 25.7 kDa |
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