pCMV6-AC-FC-S
PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal secretory signal sequence and a C-terminal murine Fc tag.
Sequence Author: OriGene
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different BsmI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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ApaI can be used between 25°C and 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different BstXI sites may not be compatible. |
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