pcDNA6.2 nLumio-DEST

Mammalian Gateway® destination vector for N-terminal tagging of a protein with the Lumio™ tetracysteine tag.

Sequence Author: Thermo Fisher (Invitrogen)

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SgrDI (6794) AhdI (5873) PciI (4983) BspQI - SapI (4867) PfoI (4407) BlpI (4311) PpuMI (4075) BbsI (4001) BclI * (3838) AvrII (3787) StuI (3786) MfeI (161) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) ATG tetracysteine tag NotI (1137) lac UV5 promoter BspEI (1453) EcoRI (1457) BsmBI - Esp3I (1681) PflMI * (1689) BssHII (1951) SgrAI (1957) NheI (1963) BmtI (1967) BbvCI (2161) SrfI (2307) BmgBI (2341) BstXI (2424) BfuAI - BspMI (2551) PstI (2562) PmeI (2719) CsiI - SexAI * (3554) pcDNA™6.2/nLumio™-DEST 6796 bp
SgrDI  (6794)
1 site
C G T C G A C G G C A G C T G C
AhdI  (5873)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4983)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (4867)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4867)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PfoI  (4407)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BlpI  (4311)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PpuMI  (4075)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BbsI  (4001)
1 site
G A A G A C N N C T T C T G N N ( N )