pcDNA6.2 V5-pL-DEST
Promoterless Gateway® destination vector for cloning of mammalian promoters and genes.
Sequence Author: Thermo Fisher (Invitrogen)
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Sticky ends from different BglI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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| * Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different PpuMI sites may not be compatible. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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| * Blocked by Dam methylation. |
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ApaI can be used between 25°C and 37°C. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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