pcDNA3.1 V5-His A
Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pcDNA™3.1/V5-His B or pcDNA™3.1/V5-His C.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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| * Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
| AmpR 4506 .. 5366 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4506 .. 5297 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4506 .. 5366 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 5298 .. 5366 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4506 .. 5366 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| NeoR/KanR 2205 .. 2999 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
| NeoR/KanR 2205 .. 2999 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
| ori 3747 .. 4335 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3747 .. 4335 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1367 .. 1795 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1367 .. 1795 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| SV40 promoter 1809 .. 2138 = 330 bp SV40 enhancer and early promoter |
| SV40 promoter 1809 .. 2138 = 330 bp SV40 enhancer and early promoter |
| bGH poly(A) signal 1097 .. 1321 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 1097 .. 1321 = 225 bp bovine growth hormone polyadenylation signal |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 3175 .. 3296 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 3175 .. 3296 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 5367 .. 5471 = 105 bp |
| AmpR promoter 5367 .. 5471 = 105 bp |
| MCS 902 .. 999 = 98 bp multiple cloning site |
| MCS 902 .. 999 = 98 bp multiple cloning site |
| V5 tag 1000 .. 1041 = 42 bp 14 amino acids = 1.4 kDa Product: epitope tag from simian virus 5 |
| V5 tag 1000 .. 1041 = 42 bp 14 amino acids = 1.4 kDa Product: epitope tag from simian virus 5 |
| 6xHis 1051 .. 1068 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 1051 . |