pCMV6-AC-mBFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal TagBFP tag.

Sequence Author: OriGene

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AanI (6523) XmnI (5845) ScaI (5726) PciI (4353) BstZ17I (3974) BsmI (3922) AanI (3861) BstBI (3684) PflFI - Tth111I (3120) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - XhoI (1082) PasI (1306) Bsu36I (1341) PshAI (1715) PmeI (1784) FseI (1794) SacII (1798) AleI (1884) XL39 (reverse primer) (1890 .. 1909) EcoO109I (2014) BsgI (2068) BbvCI - Bpu10I (2110) BlpI (2154) BstXI (2261) AgeI (2372) PflMI (2375) XcmI (2467) SexAI * (2559) SfiI (2745) AvrII (2792) BclI * (2843) BsaBI * (2861) KasI (3001) NarI (3002) SfoI (3003) PluTI (3005) PstI (3055) pCMV6-AC-mBFP 6595 bp
AanI  (6523)
2 sites
T T A T A A A A T A T T
XmnI  (5845)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5726)
1 site
A G T A C T T C A T G A
PciI  (4353)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3974)
1 site
G T A T A C C A T A T G
BsmI  (3922)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3861)
2 sites
T T A T A A A A T A T T
BstBI  (3684)
1 site
T T C G A A A A G C T T
PflFI  (3120)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3120)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
AsiSI  (1024)
1 site
G C G A T C G C C G C T A G C G
SgfI  (1024)
1 site
G C G A T C G C C G C T A G C G
MreI  (1026)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (1026)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1030)
1 site
G G C G C G C C C C G C G C G G
HindIII  (1044)
1 site
A A G C T T T T C G A A
RsrII  (1061)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (1067)
1 site
A C G C G T T G C G C A
BsiWI  (1070)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
NotI  (1076)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1082)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1082)
1 site
C T C G A G G A G C T C
PasI  (1306)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
Bsu36I  (1341)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PshAI  (1715)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PmeI  (1784)
1 site
G T T T A A A C C A A A T T T G
FseI  (1794)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SacII  (1798)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (1884)
1 site
C A C N N N N G T G G T G N N N N C A C
EcoO109I  (2014)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsgI  (2068)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbvCI  (2110)
1 site
C C T C A G C G G A G T C G
Bpu10I  (2110)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recog