pCTAP-A
Vector for expressing proteins C-terminally tagged with streptavidin- and calmodulin-binding peptides. For other reading frames, use pCTAP-B or pCTAP-C.
Sequence Author: Agilent Technologies
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
| ||
PciI is inhibited by nonionic detergents. |
|
| ||
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
| ||
Sticky ends from different PfoI sites may not be compatible. |
|
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
| ||
Sticky ends from different BsrDI sites may not be compatible. |
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
|
| ||
Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
|
| ||
Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
|
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
| ||
Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
|
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the SacII recognition sequence. |
| ||
Sticky ends from different BstXI sites may not be compatible. |
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
|
| ||
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
|
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
| ||
Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
|
|
| ||
Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
| ||
Sticky ends from different Esp3I sites may not be compatible. |
|
| ||
ApaI can be used between 25°C and 37°C. |
|
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
|
|