pcDNA4 HisMax A
Mammalian vector for high-level expression of proteins with a cleavable N-terminal 6xHis tag. For other reading frames, use pcDNA™4/HisMax B or pcDNA™4/HisMax C.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Sticky ends from different BlpI sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
| AmpR 4262 .. 5122 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4262 .. 5053 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4262 .. 5122 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 5054 .. 5122 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 4262 .. 5122 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 3503 .. 4091 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3503 .. 4091 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1541 .. 1969 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1541 .. 1969 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
| BleoR 2427 .. 2801 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| BleoR 2427 .. 2801 = 375 bp 124 amino acids = 13.8 kDa Product: antibiotic-binding protein confers resistance to bleomycin, phleomycin, and Zeocin™ |
| SV40 promoter 1983 .. 2313 = 331 bp SV40 enhancer and early promoter |
| SV40 promoter 1983 .. 2313 = 331 bp SV40 enhancer and early promoter |
| bGH poly(A) signal 1271 .. 1495 = 225 bp bovine growth hormone polyadenylation signal |
| bGH poly(A) signal 1271 .. 1495 = 225 bp bovine growth hormone polyadenylation signal |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| QBI SP163 917 .. 1079 = 163 bp strong translational enhancer derived from the 5' UTR of the mouse VEGF gene |
| QBI SP163 917 .. 1079 = 163 bp strong translational enhancer derived from the 5' UTR of the mouse VEGF gene |
| SV40 poly(A) signal 2931 .. 3052 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 2931 .. 3052 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 5123 .. 5227 = 105 bp |
| AmpR promoter 5123 .. 5227 = 105 bp |
| ATG 1080 .. 1082 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| ATG 1080 .. 1082 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| 6xHis 1092 .. 1109 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 1092 .. 1109 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| Xpress™ tag 1149 .. 1172 = 24 bp 8 amino acids = 998.0 Da 2 segments Segment 1: 1149 .. 1157 = 9 bp 3 amino acids = 409.4 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site |
| Xpress™ tag 1149 .. 1172 = 24 bp 8 amino acids = 998.0 Da 2 segments Segment 2: enterokinase site 1158 .. 1172 = 15 bp 5 amino acids = 606.5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site |
| Xpress™ tag 1149 .. 1172 = 24 bp 8 amino acids = 998.0 Da 2 segments Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site |
| EM7 promoter 2361 .. 2408 = 48 bp synthetic bacterial promoter |
| EM7 promoter 2361 .. 2408 = 48 bp synthetic bacterial promoter |
| T7 promoter 863 .. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| T7 promoter 863 .. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| SV40 ori 2164 .. 2299 = 136 bp SV40 origin of replication |
| SV40 ori 2164 .. 2299 = 136 bp SV40 origin of replication |
| MCS 1172 .. 1245 = 74 bp multiple cloning site |
| MCS 1172 .. 1245 = 74 bp multiple cloning site |
| ORF: 1492 .. 1758 = 267 bp ORF: 88 amino acids = 9.3 kDa |
| ORF: 2427 .. 2801 = 375 bp ORF: 124 amino acids = 13.8 kDa |
| ORF: 4392 .. 4658 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 2420 .. 2707 = 288 bp ORF: 95 amino acids = 10.1 kDa |
| ORF: 4262 .. 5122 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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