pTRE3G

Vector for doxycycline-inducible expression of a gene in cells containing the Tet-On® 3G transactivator protein.

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
EcoRI (3426) AatII (3355) ZraI (3353) XmnI (3032) ScaI (2913) TatI (2911) FspI (2655) NmeAIII (2581) BglI (2553) BsrFI (2513) BsaI (2494) BmrI (2473) AhdI (2433) AlwNI (1956) PspFI (1848) BseYI (1844) AvaI - BsoBI - PaeR7I - PspXI - XhoI (1) HindIII (253) KasI (274) NarI (275) SfoI (276) PluTI (278) Eco53kI (292) SacI (294) SalI (383) AccI (384) PspOMI (393) ApaI (397) BglII (399) BspDI * - ClaI * (406) EagI (411) EcoRV (419) MluI (423) NdeI (430) NheI (435) BmtI (439) PstI - SbfI (445) BamHI (447) BtgZI (484) PflMI (658) BseRI (764) StyI (806) BsgI (890) BsaBI * (1121) MfeI (1209) HpaI (1222) BspQI - SapI (1424) PciI (1540) NspI (1544) DrdI (1648) pTRE3G 3431 bp
EcoRI  (3426)
1 site
G A A T T C C T T A A G
AatII  (3355)
1 site
G A C G T C C T G C A G
ZraI  (3353)
1 site
G A C G T C C T G C A G
XmnI  (3032)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2913)
1 site
A G T A C T T C A T G A
TatI  (2911)
1 site
W G T A C W W C A T G W
FspI  (2655)
1 site
T G C G C A A C G C G T
NmeAIII  (2581)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2553)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2513)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2494)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (2473)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (2433)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1956)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1848)
1 site
C C C A G C G G G T C G
BseYI  (1844)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1)
1 site
C T C G A G G A G C T C
HindIII  (253)
1 site
A A G C T T T T C G A A
KasI  (274)
1 site
G G C G C C C C G C G G
NarI  (275)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (276)
1 site
G G C G C C C C G C G G
PluTI  (278)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
Eco53kI  (292)
1 site
G A G C T C C T C G A G
SacI  (294)
1 site
G A G C T C C T C G A G
SalI  (383)
1 site
G T C G A C C A G C T G
AccI  (384)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PspOMI  (393)
1 site
G G G C C C C C C G G G
ApaI  (397)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BglII  (399)
1 site
A G A T C T T C T A G A
BspDI  (406)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (406)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
EagI  (411)
1 site
C G G C C G G C C G G C
EcoRV  (419)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
MluI  (423)
1 site
A C G C G T T G C G C A
NdeI  (430)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (435)
1 site
G C T A G C C G A T C G
BmtI  (439)
1 site
G C T A G C C G A T C G
PstI  (445)
1 site
C T G C A G G A C G T C
SbfI  (445)
1 site
C C T G C A G G G G A C G T C C
BamHI  (447)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (484)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PflMI  (658)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BseRI  (764)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StyI  (806)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsgI  (890)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaBI  (1121)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MfeI  (1209)
1 site
C A A T T G G T T A A C
HpaI  (1222)
1 site
G T T A A C C A A T T G
BspQI  (1424)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1424)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (1540)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
NspI  (1544)
1 site
R C A T G Y Y G T A C R
DrdI  (1648)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AmpR
2360 .. 3220  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2360 .. 3151  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2360 .. 3220  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3152 .. 3220  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2360 .. 3220  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1601 .. 2189  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1601 .. 2189  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
TRE3G promoter
4 .. 382  =  379 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G
TRE3G promoter
4 .. 382  =  379 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G
SV40 poly(A) signal
1223 .. 1344  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1223 .. 1344  =  122 bp
SV40 polyadenylation signal
AmpR promoter
3221 .. 3325  =  105 bp
AmpR promoter
3221 .. 3325  =  105 bp
MCS
383 .. 452  =  70 bp
multiple cloning site
MCS
383 .. 452  =  70 bp
multiple cloning site
small t intron
583 .. 648  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
583 .. 648  =  66 bp
simian virus 40 (SV40) small t antigen intron
tet operator
12 .. 30  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
12 .. 30  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
48 .. 66  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
48 .. 66  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
84 .. 102  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
84 .. 102  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
120 .. 138  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
120 .. 138  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
156 .. 174  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
156 .. 174  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
192 .. 210  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
192 .. 210  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
228 .. 246  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
228 .. 246  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  727 .. 1200  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  35 .. 301  =  267 bp
ORF:  88 amino acids  =  10.5 kDa
ORF:  2490 .. 2756  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2360 .. 3220  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pTRE3G.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps