pTRE-Cycle1
Bidirectional expression vector for cycling the level of a protein by alternating expression and rapid degradation.
Sequence Author: Clontech (TaKaRa)
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present. Sticky ends from different EarI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different StyI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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