pTRE3G-BI-Het1

Mammalian vector for doxycycline-inducible dual expression of FKBP- and FRB-tagged proteins, allowing drug-inducible heterodimerization.

Sequence Author: Clontech (TaKaRa)

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Acc65I (3502) ATG NcoI - StyI (3498) EcoNI (3454) SphI (3387) MluI (3205) 3' MCS-2 PstI (3203) PshAI (3184) NdeI (3183) AatII (2984) ZraI (2982) SspI (2866) XmnI (2661) ScaI (2542) PvuI (2432) FspI (2284) AseI (2234) NmeAIII (2210) BglI (2182) BsaI (2123) AhdI (2062) KpnI (3506) EcoRI (3514) PaeR7I - PspXI - XhoI (70) HindIII (322) KasI (343) NarI (344) SfoI (345) PluTI (347) SalI (452) AccI (453) BglII (468) BamHI (480) ATG PaqCI (491) BsmBI - Esp3I (517) BbsI (779) BspDI * - ClaI * (817) EagI - NotI (825) MreI - NgoMIV - SgrAI (830) NaeI (832) EcoRV (850) PciI (1169) DrdI (1277) pTRE3G-BI-Het1 3524 bp
Acc65I  (3502)
1 site
G G T A C C C C A T G G
NcoI  (3498)
1 site
C C A T G G G G T A C C
StyI  (3498)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EcoNI  (3454)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SphI  (3387)
1 site
G C A T G C C G T A C G
MluI  (3205)
1 site
A C G C G T T G C G C A
PstI  (3203)
1 site
C T G C A G G A C G T C
PshAI  (3184)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NdeI  (3183)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AatII  (2984)
1 site
G A C G T C C T G C A G
ZraI  (2982)
1 site
G