pTRE3G-BI-Het1

Mammalian vector for doxycycline-inducible dual expression of FKBP- and FRB-tagged proteins, allowing drug-inducible heterodimerization.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsMammalian Expression VectorspTRE3G-BI-Het1
Acc65I (3502) ATG NcoI - StyI (3498) EcoNI (3454) SphI (3387) MluI (3205) 3' MCS-2 PstI (3203) PshAI (3184) NdeI (3183) AatII (2984) ZraI (2982) SspI (2866) XmnI (2661) ScaI (2542) PvuI (2432) FspI (2284) AseI (2234) NmeAIII (2210) BglI (2182) BsaI (2123) AhdI (2062) KpnI (3506) EcoRI (3514) PaeR7I - PspXI - XhoI (70) HindIII (322) KasI (343) NarI (344) SfoI (345) PluTI (347) SalI (452) AccI (453) BglII (468) BamHI (480) ATG PaqCI (491) BsmBI - Esp3I (517) BbsI (779) BspDI * - ClaI * (817) EagI - NotI (825) MreI - NgoMIV - SgrAI (830) NaeI (832) EcoRV (850) PciI (1169) DrdI (1277) pTRE3G-BI-Het1 3524 bp
Acc65I  (3502)
1 site		 G G T A C C C C A T G G
NcoI  (3498)
1 site		 C C A T G G G G T A C C
StyI  (3498)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
EcoNI  (3454)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SphI  (3387)
1 site		 G C A T G C C G T A C G
MluI  (3205)
1 site		 A C G C G T T G C G C A
PstI  (3203)
1 site		 C T G C A G G A C G T C
PshAI  (3184)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NdeI  (3183)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AatII  (2984)
1 site		 G A C G T C C T G C A G
ZraI  (2982)
1 site		 G A C G T C C T G C A G
SspI  (2866)
1 site		 A A T A T T T T A T A A
XmnI  (2661)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (2542)
1 site		 A G T A C T T C A T G A
PvuI  (2432)
1 site		 C G A T C G G C T A G C
FspI  (2284)
1 site		 T G C G C A A C G C G T
AseI  (2234)
1 site		 A T T A A T T A A T T A
NmeAIII  (2210)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2182)
1 site		 G C C N N N N N G G C C G G