pcDNA6 myc-His A
Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. For other reading frames, use pcDNA™6/myc-His B or pcDNA™6/myc-His C.
Sequence Author: Thermo Fisher (Invitrogen)
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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* Blocked by Dam methylation. |
AmpR 4130 .. 4990 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4130 .. 4921 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 4130 .. 4990 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4922 .. 4990 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 4130 .. 4990 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 3371 .. 3959 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 3371 .. 3959 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1358 .. 1786 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1358 .. 1786 = 429 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
BSD 2243 .. 2641 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
BSD 2243 .. 2641 = 399 bp 132 amino acids = 13.7 kDa Product: blasticidin S deaminase confers resistance to blasticidin |
CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 235 .. 614 = 380 bp human cytomegalovirus immediate early enhancer |
SV40 promoter 1800 .. 2129 = 330 bp SV40 enhancer and early promoter |
SV40 promoter 1800 .. 2129 = 330 bp SV40 enhancer and early promoter |
bGH poly(A) signal 1088 .. 1312 = 225 bp bovine growth hormone polyadenylation signal |
bGH poly(A) signal 1088 .. 1312 = 225 bp bovine growth hormone polyadenylation signal |
CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 615 .. 818 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 2799 .. 2920 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 2799 .. 2920 = 122 bp SV40 polyadenylation signal |
AmpR promoter 4991 .. 5095 = 105 bp |
AmpR promoter 4991 .. 5095 = 105 bp |
MCS 902 .. 999 = 98 bp multiple cloning site |
MCS 902 .. 999 = 98 bp multiple cloning site |
EM7 promoter 2177 .. 2224 = 48 bp synthetic bacterial promoter |
EM7 promoter 2177 .. 2224 = 48 bp synthetic bacterial promoter |
T7 promoter 863 .. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 863 .. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase |
SV40 ori 1980 .. 2115 = 136 bp SV40 origin of replication |
SV40 ori 1980 .. 2115 = 136 bp SV40 origin of replication |
Myc 997 .. 1026 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
Myc 997 .. 1026 = 30 bp 10 amino acids = 1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag |
6xHis 1042 .. 1059 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
6xHis 1042 .. 1059 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
ORF: 1309 .. 1575 = 267 bp ORF: 88 amino acids = 9.3 kDa |
ORF: 2243 .. 2641 = 399 bp ORF: 132 amino acids = 13.7 kDa |
ORF: 4260 .. 4526 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 2189 .. 2545 = 357 bp ORF: 118 amino acids = 12.8 kDa |
ORF: 4130 .. 4990 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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