pcDNA-DEST47

Gateway® destination vector for expressing C-terminally GFP-tagged proteins in mammalian cells.

Sequence Author: Thermo Fisher (Invitrogen)

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SgrDI (7778) PvuI (7227) AhdI (6857) PciI (5964) PfoI (5388) RsrII (5127) PflFI - Tth111I (4729) PluTI (4614) SfoI (4612) NarI (4611) KasI (4610) AvrII (4401) StuI (4400) BseRI (4397) CsiI - SexAI * (4168) BglII (12) SpeI (249) CMV enhancer SnaBI (590) NotI (1040) lac UV5 promoter EcoRI (1360) BsmBI - Esp3I (1584) PasI - PflMI * (1592) BbvCI (2084) SrfI (2230) BmgBI (2264) BstXI (2347) NheI (2631) BmtI (2635) PaeR7I - PspXI - XhoI (3051) BstBI (3252) BbsI (3564) DraIII (3878) pcDNA™-DEST47 7780 bp
SgrDI  (7778)
1 site
C G T C G A C G G C A G C T G C
PvuI  (7227)
1 site
C G A T C G G C T A G C
AhdI  (6857)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5964)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PfoI  (5388)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (5127)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4729)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4729)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.