Sticky ends from different BspQI sites may not be compatible.
SapI (5412) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PfoI (4952) 1 site
TCCNGGAAGGNCCT
Sticky ends from different PfoI sites may not be compatible.
BlpI (4856) 1 site
GCTNAGCCGANTCG
Sticky ends from different BlpI sites may not be compatible.
BsgI (4648) 1 site
GTGCAG(N)14NNCACGTC(N)14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
PpuMI (4620) 1 site
RGGWCCYYCCWGGR
Sticky ends from different PpuMI sites may not be compatible.