pcDNA6.2 V5-DEST

Gateway® destination vector for high-level expression of C-terminally V5-tagged proteins in mammalian cells.

Sequence Author: Thermo Fisher (Invitrogen)

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SgrDI (7339) PciI (5528) BspQI - SapI (5412) PfoI (4952) BlpI (4856) BsgI (4648) PpuMI (4620) EagI (4577) BbsI (4546) BclI * (4383) AvrII (4332) StuI (4331) CsiI - SexAI * (4099) MfeI (161) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) PstI (1434) BfuAI - BspMI (1437) BstXI (1572) BmgBI (1651) SrfI (1685) BbvCI (1828) BssHII (2058) BamHI (2099) EcoRI (2552) BspEI (2556) XbaI * (3179) PspOMI (3185) ApaI (3189) SacII (3192) BstBI (3196) AgeI (3246) PmeI (3264) pcDNA™6.2/V5-DEST 7341 bp
SgrDI  (7339)
1 site
C G T C G A C G G C A G C T G C
PciI  (5528)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5412)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5412)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PfoI  (4952)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BlpI  (4856)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsgI  (4648)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PpuMI  (4620)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EagI  (4577)
1 site
C G G C C G G C C G G C
BbsI  (4546)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BclI  (4383)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AvrII  (4332)
1 site
C C T A G G G G A T C C
StuI  (4331)
1 site
A G G C C T T C C G G A
CsiI  (4099)
1 site
A C C W G G T T