pM-secSUMOstar

Mammalian cell vector for expression of secreted recombinant proteins with a cleavable 6xHis-SUMOstar tag.

Sequence Author: LifeSensors

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SgrDI (5862) SspI (5745) ScaI (5421) PvuI (5311) AhdI (4941) PciI (4048) BstZ17I (3669) BsmI (3617) PfoI (3472) RsrII (3211) BssHII (3092) PflFI - Tth111I (2813) MfeI (161) Bpu10I (180) SpeI (249) NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) HindIII (902) 6xHis KflI - PpuMI (996) SbfI (1005) BlpI (1029) BsmBI (1290) BsmBI (1314) NotI (1321) Acc65I (1328) KpnI (1332) PaeR7I - PspXI - XhoI (1333) PspOMI (1345) ApaI (1349) BstBI (1356) AgeI (1406) 6xHis PmeI (1436) BbsI (1647) DraIII (1961) SexAI * (2252) StuI (2484) AvrII (2485) TspMI - XmaI (2506) SmaI (2508) BsaBI * (2554) KasI (2694) NarI (2695) SfoI (2696) PluTI (2698) MscI (2777) pM-secSUMOstar 5864 bp
SgrDI  (5862)
1 site
C G T C G A C G G C A G C T G C
SspI  (5745)
1 site
A A T A T T T T A T A A
ScaI  (5421)
1 site
A G T A C T T C A T G A
PvuI  (5311)
1 site
C G A T C G G C T A G C
AhdI  (4941)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4048)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3669)
1 site
G T A T A C C A T A T G
BsmI  (3617)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (3472)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3211)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (3092)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (2813)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2813)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MfeI  (161)
1 site
C A A T T G G T T A A C
Bpu10I  (180)
1 site
C C T N A G C