pTRE3G-mCherry
Vector for doxycycline-inducible expression of a gene together with the mCherry fluorescent protein.
Sequence Author: Clontech (TaKaRa)
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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FseI gradually loses activity when stored at -20°C. |
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ApaI can be used between 25°C and 37°C. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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Sticky ends from different DraIII sites may not be compatible. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C. |
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| * Blocked by Dam methylation. |
| AmpR 3644 .. 4504 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 3644 .. 4435 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 3644 .. 4504 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4436 .. 4504 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 3644 .. 4504 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| mCherry 389 .. 1099 = 711 bp 236 amino acids = 26.7 kDa Product: monomeric derivative of DsRed fluorescent protein mammalian codon-optimized |
| mCherry 389 .. 1099 = 711 bp 236 amino acids = 26.7 kDa Product: monomeric derivative of DsRed fluorescent protein mammalian codon-optimized |
| ori 2885 .. 3473 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 2885 .. 3473 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| IRES2 1107 .. 1694 = 588 bp 3 segments Segment 1: 1107 .. 1682 = 576 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) |
| IRES2 1107 .. 1694 = 588 bp 3 segments Segment 2: ATG 1683 .. 1685 = 3 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) |
| IRES2 1107 .. 1694 = 588 bp 3 segments Segment 3: 1686 .. 1694 = 9 bp internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) |
| IRES2 1107 .. 1694 = 588 bp 3 segments internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) |
| TRE3G promoter 4 .. 382 = 379 bp 3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G |
| TRE3G promoter 4 .. 382 = 379 bp 3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G |
| SV40 poly(A) signal 2507 .. 2628 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 2507 .. 2628 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 4505 .. 4609 = 105 bp |
| AmpR promoter 4505 .. 4609 = 105 bp |
| small t intron 1867 .. 1932 = 66 bp simian virus 40 (SV40) small t antigen intron |
| small t intron 1867 .. 1932 = 66 bp simian virus 40 (SV40) small t antigen intron |
| MCS 1701 .. 1736 = 36 bp multiple cloning site |
| MCS 1701 .. 1736 = 36 bp multiple cloning site |
| tet operator 12 .. 30 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 12 .. 30 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 48 .. 66 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 48 .. 66 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 84 .. 102 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 84 .. 102 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 120 .. 138 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 120 .. 138 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 156 .. 174 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 156 .. 174 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 192 .. 210 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 192 .. 210 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 228 .. 246 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| tet operator 228 .. 246 = 19 bp bacterial operator O2 for the tetR and tetA genes |
| ATG 1683 .. 1685 = 3 bp 1 amino acid = 149.2 Da Product: start codon for translation from IRES2 |
| ATG 1683 .. 1685 = 3 bp 1 amino acid = 149.2 Da Product: start codon for translation from IRES2 |
| ORF: 2011 .. 2484 = 474 bp ORF: 157 amino acids = 18.2 kDa |
| ORF: 35 .. 301 = 267 bp ORF: 88 amino acids = 10.5 kDa |
| ORF: 389 .. 1099 = 711 bp ORF: 236 amino acids = 26.7 kDa |
| ORF: 3774 .. 4040 = 267 bp ORF: 88 amino acids = 9.2 kDa |
| ORF: 3644 .. 4504 = 861 bp ORF: 286 amino acids = |