pCMV6-A-EM7-BSD
PrecisionShuttle™ mammalian vector for expressing an untagged ORF, with a blasticidin resistance marker that can also be used in bacteria.
Sequence Author: OriGene
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different BsmI sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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FseI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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