pTet-DualOFF
Vector for co-expressing ZsGreen1 with the Tet-Off® Advanced transcriptional activator.
Sequence Author: Clontech (TaKaRa)
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Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present. Sticky ends from different EarI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different DrdI sites may not be compatible. |
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* Blocked by Dam methylation. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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