pcDNA6.2 EmGFP-Bsd V5-DEST

Vivid Colors™ Gateway® destination vector for expressing proteins in mammalian cells, with an EmGFP-Bsd fusion as a transfection marker.

Sequence Author: Thermo Fisher (Invitrogen)

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SgrDI (8196) PciI (6382) BspQI - SapI (6266) EagI (5431) BbsI (5400) StuI (4316) SphI (4061) MfeI (161) CMV enhancer NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) PstI (1434) BfuAI - BspMI (1437) BstXI (1572) TspMI - XmaI (1683) SmaI - SrfI (1685) BbvCI (1828) BssHII (2058) BamHI (2099) EcoRI (2555) PspOMI (3188) ApaI (3192) SacII (3195) BstBI (3199) V5 tag PmeI (3267) BstAPI (3533) PaeR7I - PspXI - XhoI (4011) pcDNA™6.2/EmGFP-Bsd/V5-DEST 8198 bp
SgrDI  (8196)
1 site
C G T C G A C G G C A G C T G C
PciI  (6382)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (6266)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (6266)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
EagI  (5431)
1 site
C G G C C G G C C G G C
BbsI  (5400)
1 site
G A A G A