pCMV6-AN-FC-S

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal secretory signal sequence and murine Fc tag.

Sequence Author: OriGene

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HomePlasmidsMammalian Expression VectorspCMV6-AN-FC-S
DraIII (6467) XmnI (5914) AlwNI (4838) BstZ17I (4043) BsmI (3991) AanI (3930) BstBI (3753) AanI (6592) CMV enhancer NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) BamHI (992) Acc65I (998) KpnI (1002) PspOMI (1089) ApaI (1093) AsiSI - SgfI (1783) MreI - SgrAI (1785) AscI (1789) HindIII (1803) RsrII (1820) MluI (1826) NotI (1836) PaeR7I - PspXI - XhoI (1844) PmeI (1853) FseI (1863) SacII (1867) XL39 (reverse primer) (1959 .. 1978) BbvCI (2179) BlpI (2223) BsmBI (2243) BstXI (2330) AgeI (2441) PflMI (2444) XcmI (2536) SexAI * (2628) SfiI (2814) StuI (2860) AvrII (2861) BclI * (2912) BsaBI * (2930) KasI (3070) NarI (3071) SfoI (3072) PluTI (3074) PstI (3124) PflFI - Tth111I (3189) pCMV6-AN-FC-S 6664 bp
DraIII  (6467)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
XmnI  (5914)
1 site		 G A A N N N N T T C C T T N N N N A A G
AlwNI  (4838)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BstZ17I  (4043)
1 site		 G T A T A C C A T A T G
BsmI  (3991)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
AanI  (3930)
2 sites		 T T A T A A A A T A T T
BstBI  (3753)
1 site		 T T C G A A A A G C T T
AanI  (6592)
2 sites		 T T A T A A A A T A T T
NdeI  (592)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site		 T A C G T A A T G C A T
BamHI  (992)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site		 G G T A C C C C A T G G
KpnI  (1002)
1 site		 G G T A C C C C A T G G
PspOMI  (1089)
1 site		 G G G C C C C C C G G G
ApaI  (1093)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
AsiSI  (1783)
1 site		 G C G A T C G C C G C T A G C G
SgfI  (1783)
1 site		 G C G A T C G C C G C T A G C G
MreI  (1785)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (1785)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1789)
1 site		 G G C G C G C C C C G C G C G G
HindIII  (1803)
1 site		 A A G C T T T T C G A A
RsrII  (1820)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (1826)
1 site		 A C G C G T T G C G C A
NotI  (1836)
1 site		 G C G G C C G C C G C C G G C G
PaeR7I  (1844)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1844)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (1844)
1 site		 C T C G A G G A G C T C
PmeI  (1853)
1 site		 G T T T A A A C C A A A T T T G
FseI  (1863)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
SacII  (1867)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BbvCI  (2179)
1 site		 C C T C A G C G G A G T C G
BlpI  (2223)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BsmBI  (2243)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BstXI  (2330)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
AgeI  (2441)
1 site		 A C C G G T T G G C C A
PflMI  (2444)
1 site		 C C A N N