pCMV6-AN-FC-S

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal secretory signal sequence and murine Fc tag.

Sequence Author: OriGene

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DraIII (6467) XmnI (5914) AlwNI (4838) BstZ17I (4043) BsmI (3991) AanI (3930) BstBI (3753) PflFI - Tth111I (3189) AanI (6592) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) BamHI (992) Acc65I (998) KpnI (1002) PspOMI (1089) ApaI (1093) AsiSI - SgfI (1783) MreI - SgrAI (1785) AscI (1789) HindIII (1803) RsrII (1820) MluI (1826) NotI (1836) PaeR7I - PspXI - XhoI (1844) PmeI (1853) FseI (1863) SacII (1867) XL39 (reverse primer) (1959 .. 1978) BbvCI (2179) BlpI (2223) BsmBI (2243) BstXI (2330) AgeI (2441) PflMI (2444) XcmI (2536) SexAI * (2628) SfiI (2814) StuI (2860) AvrII (2861) BclI * (2912) BsaBI * (2930) KasI (3070) NarI (3071) SfoI (3072) PluTI (3074) PstI (3124) pCMV6-AN-FC-S 6664 bp
DraIII  (6467)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (5914)
1 site
G A A N N N N T T C C T T N N N N A A G
AlwNI  (4838)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstZ17I  (4043)
1 site
G T A T A C C A T A T G
BsmI  (3991)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3930)
2 sites
T T A T A A A A T A T T
BstBI  (3753)
1 site
T T C G A A A A G C T T
PflFI  (3189)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3189)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AanI  (6592)
2 sites
T T A T A A A A T A T T
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G T A C C C C A T G G
KpnI  (1002)
1 site
G G T A C C C C A T G G
PspOMI  (1089)
1 site
G G G C C C C C C G G G
ApaI  (1093)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AsiSI  (1783)
1 site
G C G A T C G C C G C T A G C G
SgfI  (1783)
1 site
G C G A T C G C C G C T A G C G
MreI  (1785)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (1785)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1789)
1 site
G G C G C G C C C C G C G C G G
HindIII  (1803)
1 site
A A G C T T T T C G A A
RsrII  (1820)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (1826)
1 site
A C G C G T T G C G C A
NotI  (1836)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (1844)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1844)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1844)
1 site
C T C G A G G A G C T C
PmeI  (1853)
1 site
G T T T A A A C C A A A T T T G
FseI  (1863)
1 site